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This recombinant M.tb esxB protein is an E.coli expressed protein (Full Length of Mature Protein) with N-terminal 6xHis tag and its purity is 90%+ determined by SDS-PAGE. With the appropriate cDNA and PCR methods, esxB expression plasmids can be rapidly produced. which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.which must undergo denaturation and folding cycle, can be recovered with more modest yields. Hence, using small-scale fermentations and laboratory-scale processing equipment, esxB proteins (or subdomains thereof) can usually be produced in sufficient quantities to initiate most studies including detailed structural determinations.esxB, also called CFP-10, is a Mycobacterium tuberculosis secreted antigen that contributes to viral virulence and pathogenicity. CFP-10 and ESAT-6 forms a 1:1 heterodimeric complex that plays a critical role in the pathogenesis of tuberculosis (TB). Amanda Welin et al. has shown that the ESAT-6/CFP-10 complex induces a transient release of Ca2+ from intracellular stores in human neutrophils. CFP-10 rather than ESAT-6 was responsible for triggering the Ca2+ response, in a pertussis toxin-sensitive manner, implying the participation of a GPCR.
|Purity||Greater than 90% as determined by SDS-PAGE.|
esxB; cfp10; lhp; mtsA10; MT3988ESAT-6-like protein EsxB; 10 kDa culture filtrate antigen CFP-10; Secreted antigenic protein MTSA-10
|Species||Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)|
|Target Protein Sequence||AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Protein Length||Full Length of Mature Protein|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Tris-based buffer，50% glycerol|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.
|Protein Families||WXG100 family, CFP-10 subfamily|