Recombinant Mycobacterium tuberculosis ESAT-6-like protein esxB(esxB)

In Stock
Code CSB-YP363658MVZ
Size US$1916Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names esxB
Uniprot No. P9WNK4
Research Area Others
Alternative Names esxB; cfp10; lhp; mtsA10; MT3988ESAT-6-like protein EsxB; 10 kDa culture filtrate antigen CFP-10; Secreted antigenic protein MTSA-10
Species Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Source Yeast
Expression Region 2-100aa
Target Protein Sequence AEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 12.7kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

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Applications : circular dichroism (CD) analysis

Review: circular dichroism (CD) analysis indicates structural changes due to binding interaction between capture aptamer and CFP10-ESAT6 antigen.

By Anonymous

Applications : antigen

Review: Comparison of the DPV peak currents in the presence of BSA, ESAT-6, and CFP-10 respectively. All targets are at 100 ng.m-1 . The error bars represent the standard deviation of three measurements. Blank control: 10 mM PBS (pH 7.4).

By Anonymous

Target Background

Function
A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.
Subcellular Location Secreted.
Protein Families WXG100 family, CFP-10 subfamily
Database Links

KEGG: mtc:MT3988

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