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The cDNA fragment encoding 24-228aa of Mycobacterium tuberculosis immunogenic protein MPT64 was fused with an N-terminal 6xHis-tag and then expressed in the yeast cells. The product obtained is the recombinant full-length of mature Mycobacterium tuberculosis MPT64 protein. Its purity was determined using SDS-PAGE and reached up to 90%. Under the reducing conditions, the gel presented a molecular mass band of about 21-24 kDa. It also underwent the validation through the LC-MS/MS analysis. In-stock MPT64 proteins are offered now. This recombinant MPT64 protein may find uses in the specific antibody production or the studies of tuberculosis (TB) infection.
The level of expression of the MPT64 protein is high in patients with active TB. Both in sputum and serum exosomes of a TB infected patient can be detected the presence of MPT64. A special receptor, single-stranded DNA (ssDNA) aptamer, can specifically recognize MPT64 protein. MPT64 mainly promotes survival and persistence in the host cell. It deactivates the expression of apoptotic cytokines and plays a major role in the survival and virulence of the mycobacteria.
|Purity||Greater than 90% as determined by SDS-PAGE.|
mpt64; MT2032; Immunogenic protein MPT64; Antigen MPT64
|Species||Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)|
|Target Protein Sequence||APKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYIAQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRKPITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEAAGPTQVLVPRSAIDSMLA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Protein Length||Full Length of Mature Protein|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Tris-based buffer,50% glycerol|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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|Gene References into Functions||