mpt64 Antibody

Code CSB-PA14949A0Rb
Size US$299
Image
  • Western Blot
    Positive WB detected in: mpt64 293T Transfected lysate, 293T non-Transfected lysate
    All lanes: mpt64 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 51 kDa
    Observed band size: 51 kDa
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Product Details

Description
mpt64 polyclonal antibody CSB-PA14949A0Rb was produced in the rabbits immunized by using the Recombinant Mycobacterium tuberculosis Immunogenic protein MPT64 protein (24-228AA) as the immunogen. The target protein mpt64 is a 23 kDa secreted protein restricted to members of the Mycobacterium tuberculosis complex (MTBC) which elicits T cell responses and cutaneous DTH reactions in Myco. tuberculosis-infected animals. This protein is recognized by human Th1 cells, and thus it could be useful for TB diagnosis or as part of a novel candidate vaccine against Tuberculosis (TB).
This Rabbit anti-Mycobacterium tuberculosis mpt64 Polyclonal antibody was tested in the ELISA and WB applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the mpt64 proteins of Mycobacterium tuberculosis-origin and may be used to detect the endogenous levels of mpt64 protein.
Full Product Name Rabbit anti-Mycobacterium tuberculosis mpt64 Polyclonal antibody
Uniprot No. P9WIN9
Target Names mpt64
Alternative Names mpt64 antibody; Rv1980c antibody; MTCY39.39 antibody; Immunogenic protein MPT64 antibody; Antigen MPT64 antibody
Raised in Rabbit
Species Reactivity Mycobacterium tuberculosis
Immunogen Recombinant Mycobacterium tuberculosis Immunogenic protein MPT64 protein (24-228AA)
Immunogen Species Mycobacterium tuberculosis
Conjugate Non-conjugated

The mpt64 Antibody (Product code: CSB-PA14949A0Rb) is Non-conjugated. For mpt64 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA14949B0Rb mpt64 Antibody, HRP conjugated ELISA
FITC CSB-PA14949C0Rb mpt64 Antibody, FITC conjugated
Biotin CSB-PA14949D0Rb mpt64 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
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Applications : /

Sample type: cells

Review: MPT64 and CFP10 peroxidase-labeled secondary antibodies were purchased from Cusabio (Wuhan, China).The optimization of concentration and incubation time for the secondary HRP-antibodies for (a,b) WE1 (CFP10) and (c,d) WE2 (MPT64) (n ≥ 3).

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Target Background

Gene References into Functions
  1. Aptamers against Mtb secreted protein MPT64 were successfully selected and could be used as an alternative for detection and diagnosis of tuberculosis. PMID: 28454652
  2. we found that polymorphisms of the mpt64 gene in the MTBC may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion. PMID: 23390287
  3. CFP21 (cutinase precursor) and MPT64 fusion protein stimulate higher level of interferon (IFN)-gamma in tuberculin skin test (TST)-positive healthy population than in TST-negative healthy population. PMID: 21340709
  4. Data show that Recombinant Mycobacterium vaccae secreted MPT64 could induce high level humoral and cell mediated immune responses in mice and could be used as a candidate of new vaccine against TB. PMID: 19257983
  5. Recombinant rMPT64 elicits a specific immune response indistinguishable from that of MPB64 purified from BCG Tokyo culture filtrates. PMID: 16216527

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Subcellular Location Secreted.
Database Links

KEGG: mtu:Rv1980c

STRING: 83332.Rv1980c

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