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Recombinant Mycoplasma pneumoniae Probable cytosol aminopeptidase (pepA)

Recombinant Mycoplasma pneumoniae Probable cytosol aminopeptidase (pepA)

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Code	CSB-EP302719MLW
Abbreviation	Recombinant Mycoplasma pneumoniae pepA protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302719MLW could indicate that this peptide derived from E.coli-expressed Mycoplasma pneumoniae (strain ATCC 29342 / M129) pepA. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302719MLW could indicate that this peptide derived from E.coli-expressed Mycoplasma pneumoniae (strain ATCC 29342 / M129) pepA.
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

pepA

Uniprot No.

P75206

Research Area

Microbiology

Alternative Names

pepA; MPN_572; MP270Probable cytosol aminopeptidase; EC 3.4.11.1; Leucine aminopeptidase; LAP; EC 3.4.11.10; Leucyl aminopeptidase

Species

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Source

E.coli

Expression Region

1-445aa

Target Protein Sequence

MKLNKLFDSKTVVFKKSDKYGKSDCCKTKCVEGQIEFGVKIPTDFPAFNRALVTFLKTQKNQLNVDLDSFVELYKAENLCYKTALKVVVASITFCETTPFTMKTEPQKNVEVAVKCDSEHTSLIKEYEVVGNYVNMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPVWRLPMHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTLYERAAQQAKE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

64.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Constructing a plasmid that codes for the Mycoplasma pneumoniae (strain ATCC 29342 / M129) pepA protein (1-445aa) initiates the generation of the recombinant Mycoplasma pneumoniae (strain ATCC 29342 / M129) pepA protein. The constructed plasmid is transformed into 1-445aa cells. Positive e.coli cells are selected and then cultured under conditions that encourage the expression of the gene of interest. The protein is equipped with a N-terminal 6xHis-SUMO tag. After that, affinity purification is employed to isolate and purify the recombinant pepA protein from the cell lysate. The purity of the resulting recombinant pepA protein is over 90%, determined by SDS-PAGE.

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Target Background

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

Subcellular Location

Cytoplasm.

Protein Families

Peptidase M17 family

Database Links

KEGG: mpn:MPN572

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