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Recombinant Paenibacillus macerans Cyclomaltodextrin glucanotransferase, partial

Recombinant Paenibacillus macerans Cyclomaltodextrin glucanotransferase, partial

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Code	CSB-EP327084EQJ
MSDS	MSDS
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Uniprot No.

P31835

Research Area

Microbiology

Alternative Names

; Cyclomaltodextrin glucanotransferase; EC 2.4.1.19; Cyclodextrin-glycosyltransferase; CGTase

Species

Paenibacillus macerans (Bacillus macerans)

Source

E.coli

Expression Region

608-713aa

Target Protein Sequence

VLTADQVTVRFKVNNATTALGQNVYLTGNVAELGNWTAANAIGPMYNQVEASYPTWYFDVSVPANTALQFKFIKVNGSTVTWEGGNNHTFTSPSSGVATVTVDWQN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

15.4kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I have a question regarding CSB-EP327084EQJ - Cyclomaltodextrin glucanotransferase, partial Recombinant Protein (Bacillus macerans). Do you have any information on the activity of this enzyme for the enzyme mediated synthesis of ascorbic acid 2-glucoside? Do you have any activity assay data?

Very nice to receive your inquiry.
We haven't tested the activity of this protein so can't 100% guarantee that it is active. The expression region we provide is CBM20 domain.
The protein is purified under mild conditions which should have activity in theory.