Name: Recombinant Pig Cholesterol 7-alpha-monooxygenase (CYP7A1), partial
Brand: CUSABIO
SKU: 006460PI

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CYP7A1

Uniprot No.

O46491

Alternative Names

CYP7A1; CYP7Cytochrome P450 7A1; 24-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.26; CYPVII; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; EC 1.14.14.23

Species

Sus scrofa (Pig)

Expression Region

1-501

Target Protein Sequence

MMSISLLGGI VTAVCCCLWL LLGMRRRQTG EPPLENGIIP YLGCALQFGA NPLEFLRANQ RKHGHIFTCQ LMGNYVHFIT NPLSYHKVLC HGKYLDWKKF HFTASAKAFG HRSIDPSDGN TTDNINKTII KTLQGDALNL LAAAMMENLQ LVLRPQVAPQ PEKPAWVTEG MYSFCYRVMF EAGYVTLFGK DPIGHDAQKA LILNNLDNFK QFDKIFPALV AGFPIHVFKT GHYAREKLAE GLRLQKLRKR DHISELVRFL NDTLSTLDDA EKAKSLLAVL WASQANTIPA TFWCLFQTIR SPEAMKAASE EVNKTLEKAG QKISLDDKPI YLNQIELDSM PVLDSIIKES LRLSSASLNI RTAKEDFTLH LQDGSYNIRK DDIIALYPQL MHLDPEIYPD PLTFKYDRYL DENGKTKTTF YSHGLKLKYY YMPFGSGATI CPGRLFAVQE IKQFLILMLS YFDLELVESH VKCPPLDQSR AGLGILPPSN DIEFRYKLKH L

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I am writing to you to check if you have any porcine CYP7A1 antibody, and recombinant CYP7A1 protein. If you can let me know size, pricing, and time frame for getting those, it will be highly appreciated.

We provide recombinant CYP7A1 proteins which are made-to-order currently.
We do not offer antibodies for porcine CYP7A1, we have the following two antibodies for detecting human CYP7A1. If you're interested in a custom antibody production, we can help generate a rabbit polyclonal antibody against the pig CYP7A protein(Uniprot No.O46491).
CSB-PA006460LA01HU CYP7A1 Antibody https://www.cusabio.com/Polyclonal-Antibody/CYP7A1-Antibody-12921505.html
CSB-PA646965 CYP7A1 Antibody https://www.cusabio.com/Polyclonal-Antibody/Rabbit-anti-human-CYP7A1-polyclonal-antibody-11105079.html

Target Background

Function

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.

Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Database Links

STRING: 9823.ENSSSCP00000006650

UniGene: Ssc.30521

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Recombinant Pig Cholesterol 7-alpha-monooxygenase (CYP7A1), partial

Product Details

Customer Reviews and Q&A

Target Background

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