Recombinant Pig Complement C5a anaphylatoxin (C5)

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Code CSB-YP003995PI
MSDS
Size $436
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP003995PI could indicate that this peptide derived from Yeast-expressed Sus scrofa (Pig) C5.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP003995PI could indicate that this peptide derived from Yeast-expressed Sus scrofa (Pig) C5.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
C5
Uniprot No.
Research Area
Others
Alternative Names
C5; Complement C5a anaphylatoxin
Species
Sus scrofa (Pig)
Source
Yeast
Expression Region
1-74aa
Target Protein Sequence
MLQKKIEEEAAKYKYAMLKKCCYDGAYRNDDETCEERAARIKIGPKCVKAFKDCCYIANQVRAEQSHKNIQLGR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
10.6kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The Recombinant Pig Complement C5a anaphylatoxin (C5) is a meticulously engineered copy of the native Complement C5a anaphylatoxin from Sus scrofa (Pig). This product is specifically designed for researchers aiming to study this vital protein.

The protein is expressed using yeast as the source organism, and encompasses the full length of the protein, from 1-74aa. This results in a protein that maintains all the functional features of the native counterpart. To facilitate efficient purification and detection, the protein has been N-terminal 6xHis-tagged.

We assure the quality of Recombinant Pig Complement C5a anaphylatoxin (C5) by ensuring a purity of greater than 90%, as evidenced by SDS-PAGE. This high level of purity ensures that you receive a reliable, high-performance product for your experiments.

Customer Reviews and Q&A

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Target Background

Function
Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Subcellular Location
Secreted.
Database Links
CUSABIO guaranteed quality
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