Recombinant Pig Epididymal secretory glutathione peroxidase(GPX5)

Code CSB-BP009870PI
Size Pls inquire
Source Baculovirus
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Code CSB-EP009870PI-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP009870PI
Size Pls inquire
Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names GPX5
Uniprot No. O18994
Alternative Names GPX5Epididymal secretory glutathione peroxidase; EC 1.11.1.9; Epididymis-specific glutathione peroxidase-like protein; EGLP; Glutathione peroxidase 5; GPx-5; GSHPx-5
Species Sus scrofa (Pig)
Expression Region 22-219
Target Protein Sequence NSNLEKMDC YKDVTGTIYD YDAFTLNGNE HIQFKQYAGK HVLFVNVATY CGLTAQYPEL NTLQEELKPF GLVVLGFPCN QFGKQEPGEN SEILLGLKYV RPGGGYVPNF QLFEKGDVNG EKEQKVFTFL KHSCPHPSEL IGSIGYISWE PIRVHDIRWN FEKFLVGPDG VPVMRWVHET PISTVKSDIL AYLKQFKTE
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.
Gene References into Functions
  1. Sperm TPI content and amounts of GPX5 in seminal plasma may be used as quality markers of boar sperm. PMID: 26711247
Subcellular Location Secreted
Protein Families Glutathione peroxidase family
Tissue Specificity Proximal caput epididymis.
Database Links

KEGG: ssc:396920

STRING: 9823.ENSSSCP00000001283

UniGene: Ssc.14513

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