Recombinant Pig Interstitial collagenase (MMP1)

Code CSB-YP014656PI
MSDS
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Source Yeast
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Code CSB-EP014656PI
MSDS
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Source E.coli
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Code CSB-EP014656PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014656PI
MSDS
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Source Baculovirus
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Code CSB-MP014656PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MMP1
Uniprot No.
Alternative Names
MMP1; Interstitial collagenase; EC 3.4.24.7; Matrix metalloproteinase-1; MMP-1) [Cleaved into: 18 kDa interstitial collagenase]
Species
Sus scrofa (Pig)
Expression Region
100-469
Target Protein Sequence
F VLTPGNPRWE NTHLTYRIEN YTPDLSREDV DRAIEKAFQL WSNVSPLTFT KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED ERWTKNFRDY NLYRVAAHEL GHSLGLSHST DIGALMYPNY IYTGDVQLSQ DDIDGIQAIY GPSENPVQPS GPQTPQVCDS KLTFDAITTL RGELMFFKDR FYMRTNSFYP EVELNFISVF WPQVPNGLQA AYEIADRDEV RFFKGNKYWA VRGQDVLYGY PKDIHRSFGF PSTVKNIDAA VFEEDTGKTY FFVAHECWRY DEYKQSMDTG YPKMIAEEFP GIGNKVDAVF QKDGFLYFFH GTRQYQFDFK TKRILTLQKA NSWFNCRKN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
Gene References into Functions
  1. the identification of MMP1 and MMP10 genes in swine is reported. PMID: 19639408
  2. increased interstitial MMP1 activity during ischemia and reperfusion is a result of the endothelin protein kinase c pathway and may be due to enhanced phosphorylation of mmp1 PMID: 18065523
  3. Results indicate that leukemia inhibitory factor (LIF) and Oncostatin M increase the expression of MMP-1, MMP-3, and TIMP-1 several fold, and that their expression is reduced to basal levels in the presence of the LIF antagonist MH35-BD. PMID: 19342253
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links
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