Recombinant Escherichia coli O157:H7 Protease 7(ompT)

Code CSB-YP348292EOD
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Source Yeast
Code CSB-EP348292EOD-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP348292EOD
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Source Baculovirus
Code CSB-MP348292EOD
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names ompT
Uniprot No. P58603
Species Escherichia coli O157:H7
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Q&A and Customer Reviews


I have a question about this project.
I have checked each sequence, and what is the exact product of actual sequence of OmpT?
​Also, I would like to know why mutation is recommended, and what is the difference between each product​?

Thanks for your inquiry. The product CSB-EP348292EOD is the exact product of actual sequence of OmpT.
We recommend the mutated sequence (CSB-EP348292EOD(M)) because its lead time is relatively short. And we have customer ordered this product before.
The customer who ordered this protein before requested these two sites mutation, probably he wants to make a research on about mutation influence on the protein, the research is about avoiding the automatic hydrolysis of protein. The only difference is the remarked two sites exchange.
 Customer Reviews

Target Data

Function Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues (By similarity).
Subcellular Location Cell outer membrane, Multi-pass membrane protein
Protein Families Peptidase A26 family
Database Links

KEGG: ece:Z1931

STRING: 155864.Z1931

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