Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase (pvdA)

Code CSB-YP706553EZX
MSDS
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Source Yeast
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Code CSB-EP706553EZX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP706553EZX
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP706553EZX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pvdA
Uniprot No.
Alternative Names
pvdA; pvd-1; PA2386; L-ornithine N(5)-monooxygenase; EC 1.14.13.195; L-ornithine N(5)-hydroxylase; Ornithine hydroxylase; L-ornithine N(5)-oxygenase; Pyoverdin biosynthesis protein A
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-443
Target Protein Sequence
MTQATATAVV HDLIGVGFGP SNIALAIALQ ERAQAQGALE VLFLDKQGDY RWHGNTLVSQ SELQISFLKD LVSLRNPTSP YSFVNYLHKH DRLVDFINLG TFYPCRMEFN DYLRWVASHF QEQSRYGEEV LRIEPMLSAG QVEALRVISR NADGEELVRT TRALVVSPGG TPRIPQVFRA LKGDGRVFHH SQYLEHMAKQ PCSSGKPMKI AIIGGGQSAA EAFIDLNDSY PSVQADMILR ASALKPADDS PFVNEVFAPK FTDLIYSREH AERERLLREY HNTNYSVVDT DLIERIYGVF YRQKVSGIPR HAFRCMTTVE RATATAQGIE LALRDAGSGE LSVETYDAVI LATGYERQLH RQLLEPLAEY LGDHEIGRDY RLQTDERCKV AIYAQGFSQA SHGLSDTLLS VLPVRAEEIS GSLYQHLKPG TAARALHEHA LAS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.
Gene References into Functions
  1. PvdA is a membrane-associated protein located at the old cell pole PMID: 22498339
  2. The first two structures of a class B N-hydroxylating monooxygenase were determined with FAD in oxidized (1.9 A resolution) and reduced (3.03 A resolution) states PMID: 21757711
  3. overexpression and purification of active recombinant L-ornithine N5-hydroxylase, PvdA; detailed steady-state kinetic analysis to determine the substrate and coenzyme specificities PMID: 17015659
  4. The membrane topogenic determinants of PvdA were studies, and PvdA appears to have a single N-terminal domain anchored to the inner membrane. PMID: 18757814
  5. PvdA employs a kinetic mechanism that is a hybrid of those previously documented for other flavin-dependent hydroxylases PMID: 19368334

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Subcellular Location
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family
Database Links

KEGG: pae:PA2386

STRING: 208964.PA2386

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