pvdA Antibody

Datasheet

Code	CSB-PA706553HA01EZX
Size	US$166
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: pvdA antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Pseudomonas aeruginosa pvdA Polyclonal antibody

Uniprot No.

Q51548

Target Names

pvdA

Alternative Names

pvdA antibody; pvd-1 antibody; PA2386 antibody; L-ornithine N(5)-monooxygenase antibody; EC 1.14.13.195 antibody; L-ornithine N(5)-hydroxylase antibody; Ornithine hydroxylase antibody; L-ornithine N(5)-oxygenase antibody; Pyoverdin biosynthesis protein A antibody

Raised in

Rabbit

Species Reactivity

Pseudomonas aeruginosa

Immunogen

Recombinant Pseudomonas aeruginosa L-ornithine N(5)-monooxygenase protein (1-443AA)

Immunogen Species

Pseudomonas aeruginosa

Conjugate

Non-conjugated

The pvdA Antibody (Product code: CSB-PA706553HA01EZX) is Non-conjugated. For pvdA Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA706553HB01EZX	pvdA Antibody, HRP conjugated	ELISA
FITC	CSB-PA706553HC01EZX	pvdA Antibody, FITC conjugated
Biotin	CSB-PA706553HD01EZX	pvdA Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.

Gene References into Functions

PvdA is a membrane-associated protein located at the old cell pole PMID: 22498339
The first two structures of a class B N-hydroxylating monooxygenase were determined with FAD in oxidized (1.9 A resolution) and reduced (3.03 A resolution) states PMID: 21757711
overexpression and purification of active recombinant L-ornithine N5-hydroxylase, PvdA; detailed steady-state kinetic analysis to determine the substrate and coenzyme specificities PMID: 17015659
The membrane topogenic determinants of PvdA were studies, and PvdA appears to have a single N-terminal domain anchored to the inner membrane. PMID: 18757814
PvdA employs a kinetic mechanism that is a hybrid of those previously documented for other flavin-dependent hydroxylases PMID: 19368334

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Subcellular Location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family

Database Links

KEGG: pae:PA2386

STRING: 208964.PA2386

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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