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Recombinant Rabbit Tropomyosin alpha-1 chain (TPM1)

Recombinant Rabbit Tropomyosin alpha-1 chain (TPM1)

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Code	CSB-EP024104RB
Abbreviation	Recombinant Rabbit TPM1 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP024104RB could indicate that this peptide derived from E.coli-expressed Oryctolagus cuniculus (Rabbit) TPM1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP024104RB could indicate that this peptide derived from E.coli-expressed Oryctolagus cuniculus (Rabbit) TPM1.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TPM1

Uniprot No.

P58772

Research Area

Signal Transduction

Alternative Names

TPM1; TPMA; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1

Species

Oryctolagus cuniculus (Rabbit)

Source

E.coli

Expression Region

1-284aa

Target Protein Sequence

MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Experience a genuine copy of the Tropomyosin alpha-1 chain protein with our Recombinant Rabbit TPM1. This protein, known as Alpha-tropomyosin or Tropomyosin-1, is a pivotal player in the field of Signal Transduction. Our model protein is carefully constructed using the TPM1 (TPMA) gene from the rabbit species, expressed in E.coli. Spanning from the 1st to the 284th amino acid, it ensures the complete representation of the full-length protein.

Our Recombinant Rabbit TPM1, designed with an N-terminal 10xHis-tag and a C-terminal Myc-tag, simplifies detection and purification processes. As quality is our priority, the protein surpasses a purity level of 90% as confirmed by SDS-PAGE. Whether your research needs call for a liquid form or a lyophilized powder, we have you covered, providing a flexible solution for your research needs.

Customer Reviews and Q&A

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Target Background

Function

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Gene References into Functions

The results indicate that cross-linking significantly affects properties of Tpm and actin-myosin interaction and can explain, at least partly, the role of the interchain disulfide cross-linking of cardiac Tpm in human heart diseases. PMID: 27856252
altered TM-actin contacts destabilized the thin filament and affected the actin-myosin interactions PMID: 27480605
analysis of the thin filament associated with the R167H and K168E substitutions in tropomyosin Tpm1.1 PMID: 27956029
We propose that TR100 acts to compromise the integrity of Tpm cables rather than prevent overlap complex formation. Our data suggests that TR100 is incorporated into the growing actin-Tpm co-polymer given that its effects cannot be observed on pre-formed Tpm3.1/actin filaments PMID: 26804624
Maximal Ca(2+) activated force was the same in alphaalphaTm versus betabetaTm myofibrils, but betabetaTm myofibrils showed a marked slowing of relaxation and an impairment of regulation under resting conditions PMID: 25380572
Tmod1 and Tmod3 showed somewhat different tropomyosin-binding site utilization. PMID: 24922351
Thermal denaturation of rabbit cardiac alpha,alpha-tropomyosin is monitored at neutral pH and compared to shark tropomyosin, showing that amino acid substitutions predicted to be unfavorable in one temperature regime are desirable in another. PMID: 21707054
The rotational motion of a spin label covalently bound to the side chain of a cysteine genetically incorporated into rabbit skeletal muscle tropomyosin, was measured. PMID: 21575577
a computational search assessing electrostatic interactions for multiple azimuthal locations, z-positions, and pseudo-rotations of tropomyosin on F-actin was performed. PMID: 21320445