Recombinant Rat Calreticulin (Calr)

Code CSB-YP004458RA
MSDS
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Source Yeast
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Code CSB-EP004458RA
MSDS
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Source E.coli
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Code CSB-EP004458RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004458RA
MSDS
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Source Baculovirus
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Code CSB-MP004458RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Calr
Uniprot No.
Alternative Names
CalrCalreticulin; CALBP; CRP55; Calcium-binding protein 3; CABP3; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP
Species
Rattus norvegicus (Rat)
Expression Region
18-416
Target Protein Sequence
DPA IYFKEQFLDG DAWTNRWVES KHKSDFGKFV LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPGGLDQK DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAYDS FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT KAAEKQMKDK QDEEQRLKEE EEDKKRKEEE EAEDKEDEDD RDEDEDEEDE KEEDEEDATG QAKDEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy.
Gene References into Functions
  1. CRT might protect cardiomyocytes from apoptosis induced by angiotensin II, in which ER stress and mitochondria function are possible underlying molecular bases PMID: 29453988
  2. Exogenous CRT attenuates MR-induced ER stress-related apoptosis by suppressing CHOP-mediated apoptotic signaling pathways in MMECs. PMID: 24589181
  3. Data highlights the potential importance of CRT and other endoplasmic reticulum stress proteins in inflammatory demyelination PMID: 24252779
  4. These data demonstrate that CRT is localized at cardiomyocyte mitochondria and its mitochondrial content is increased in DCM hearts. PMID: 24813996
  5. Data suggest that Wistar Kyoto rats are unlike humans in that plasma calreticulin is not up-regulated during pregnancy in rats. Placental calreticulin is not altered by fetal growth retardation in either species (uterine vessel ligation rat model). PMID: 23972286
  6. found that at 7 days of single-prolonged stress rats had the highest calreticulin expression PMID: 23575574
  7. CRT-regulated Ca(2+)-dependent pathways are a critical molecular link between ER stress and TGF-beta fibrotic signaling PMID: 23564462
  8. The level of CRT is enhanced in nerve growth factor-stimulated differentiation of PC-12 cells through extracellular signal-regulated kinase (ERK)-dependent mitogen-activated protein kinase (MAPK) pathway. PMID: 22147490
  9. Calreticulin exits the endoplasmic reticulum (ER) to accumulate in the ER-Golgi intermediate compartment and the cis-Golgi, together with sub-optimally loaded H-2Db histocompatibility class I antigen molecules. PMID: 19851281
  10. CRT P-domain fragment CRT(221-256) constitutes an autonomous folding unit, and has a structure highly similar to that of the corresponding region in CRT(189-288) PMID: 12270713
  11. may represent a new class of high-affinity melatonin binding sites involved in some functions of the indoleamine including genomic regulation PMID: 12603316
  12. Calreticulin inhibits the MEK1,2-ERK1,2 pathway in alpha 1-adrenergic receptor/Gh-stimulated hypertrophy of neonatal rat cardiomyocytes PMID: 12648529
  13. Data show that calreticulin has an independent role in the cytosol that depends critically on its inefficient compartmentalization due to the signal sequence. PMID: 16198296
  14. CRT arginylation occurs in the cytosolic pool of mature CRT (defined by an Asp acid N-terminal) that is probably retrotranslocated from the endoplasmic reticulum PMID: 17197444
  15. Calreticulin is a high affinity ligand of GABARAP. PMID: 17916189
  16. These findings imply an important role of serine dephosphorylation and tyrosine phosphorylation on calreticulin mediated AT1 receptor mRNA stability. PMID: 18413143

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Subcellular Location
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen. Cytoplasmic vesicle, secretory vesicle, Cortical granule. Cytolytic granule.
Protein Families
Calreticulin family
Tissue Specificity
Predentin and odontoblast.
Database Links

KEGG: rno:64202

STRING: 10116.ENSRNOP00000004091

UniGene: Rn.974

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