Recombinant Rat Cytochrome b-245 light chain (Cyba)

Code CSB-YP730804RA
MSDS
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Source Yeast
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Code CSB-EP730804RA
MSDS
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Source E.coli
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Code CSB-EP730804RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730804RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP730804RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CybaCytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
Species
Rattus norvegicus (Rat)
Expression Region
2-192
Target Protein Sequence
GQIEWAMWA NEQALASGLI LITGGIVATA GRFTQWYFGA YSIVAGVLIC LLEYPRGKRK KGSTMERCGQ KYLTAVVKLF GPLTRNYYVR AVLHLLLSVP AGFLLATILG TVCLAIASVI YLLAAIRGEQ WTPIEPKPKE RPQVGGTIKQ PPTNPPPRPP AEVRKKPSEA EEEAASAGGP QVNPIPVTDE VV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Gene References into Functions
  1. p22phox has a role in sympathetic regulation of blood pressure in stroke-prone spontaneously hypertensive rats PMID: 27824157
  2. Data show that functional loss of NADPH oxidases (Nox) associated with the absence of P22PHOX protein did not mitigate the size of infarction produced by distal middle cerebral artery (MCA) occlusion. PMID: 26389812
  3. Data indicate that green tea polyphenols (GTPs) treatments protected against the high glucose (HG)-induced over-expressions of p22phox and p67phox (NCF2). PMID: 24580748
  4. Long-term treatment with sesamin improves arterial function in spontaneously hypertensive rats through downregulation of p22(phox) and p47(phox) expression and upregulation of endotheliail nitric oxide synthase 3 (eNOS) expression. PMID: 23624755
  5. Data indicate that the effects of Il33 polymorphism were manifested only in backcross rats with loss of Cyba function. PMID: 23446743
  6. Telmisartan downregulates myocardial expression of p22phox in type 2 diabetic rats. PMID: 22873349
  7. Chronic intermittent hypoxia upregulates the expression of p22phox in rat carotid body and elevates the rat systolic blood pressure. PMID: 21429415
  8. Jinmaitong-medicated serum can down-regulate the expressions of iNOS protein and NADPH oxidase p22-phox subunit mRNA of Schwann cells cultured in high-glucose medium. PMID: 21419083
  9. Angiotensin II increases ROS production in fetal rat cardiac fibroblasts probably by inducing p22phox over-expression. PMID: 19246278
  10. Enhanced expression of p22phox contributes to augmented VSMC proliferation in diabetic rats. PMID: 15036821
  11. cytochrome b558 expression is downregulated via the reduction of heme availability after NADPH oxidase is inhibited by HO-1 PMID: 15123630
  12. Hyperhomocysteinemia caused a 100% increase in the p22phox subunit of NADPH oxidase, and superoxide produced by NADPH oxidase reduces the ability of NO to regulate mitochondrial function in the myocardium PMID: 15851618
  13. the p22(phox) gene has a role in hypertension [review] PMID: 16115038
  14. expression of p22-phox mRNA in CMEC is upregulated by growth through a mechanism that involves increases in transcriptional activity of the p22-phox gene and stability of the p22-phox message after its formation PMID: 16257643
  15. An increase in p22phox is required for increased renal NADPH oxidase activity, expression of Nox proteins and oxidative stress, and contributes < or =50% to hypertension during an Ang II slow-pressor response. PMID: 16391171
  16. Increases in p22-phox mRNA levels induced by a stretch force in combination with angiotensin II were prevented by treatment with an angiotensin type I (AT1) receptor antagonist, RNH-6270 (100 nmol/l). PMID: 16685209
  17. up-regulation of NADPH oxidase subunit by anti-TGF-beta antibody strongly implicates for the first time the up-regulation of renal parenchymal cell NADPH oxidase in the aetiology of immunosuppression-induced nephrotoxicity. PMID: 17085464
  18. p22(phox) and NADPH oxidase regulate the single nephron glomerular filtration rate during ANG II infusion via macula densa-dependent mechanisms PMID: 17220186
  19. mRNA for the oxidase subunits NOX1, NOX2, NOX4, p47(phox), and p22(phox) were present in both sympathetic celiac ganglion (CG) and dorsal root ganglion (DRG), but mRNA for NOX4 was significantly higher in CG than in DRG in DOCA hypertension. PMID: 17698723
  20. Eosinophilia in the MES rat strain is caused by a loss-of-function mutation in the gene for cytochrome b(-245), alpha peptide (Cyba). PMID: 19406829
  21. Poldip2 associates with p22phox to activate Nox4, leading to regulation of focal adhesion turnover and vascular smooth muscle cell migration, thus linking reactive oxygen species production and cytoskeletal remodeling. PMID: 19574552
  22. superoxide generated by NADPH oxidase inhibits proximal tubule fluid reabsorption in spontaneously hypertensive rats PMID: 19805644

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Subcellular Location
Cell membrane.
Protein Families
P22phox family
Tissue Specificity
Expressed to a relatively high level in kidney, spleen, thymus and lung, and to a lower level in aorta, adrenals, and heart. Expression is not detected in liver or brain.
Database Links
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