Recombinant Rat Dynamin-2 (Dnm2), partial

Code CSB-YP007079RA
MSDS
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Source Yeast
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Code CSB-EP007079RA
MSDS
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Source E.coli
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Code CSB-EP007079RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007079RA
MSDS
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Source Baculovirus
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Code CSB-MP007079RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dnm2
Uniprot No.
Alternative Names
Dnm2; Dyn2Dynamin-2; EC 3.6.5.5
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.
Gene References into Functions
  1. Study is the first to show the association of dynamin Charcot-Marie-Tooth disease mutations with aberrant actin dynamics and lamellipodia, which may contribute to defective endocytosis and myelination in Schwann cells in Charcot-Marie-Tooth disease. PMID: 27328317
  2. Reduction in dynamin-2 is implicated in ischaemic cardiac arrhythmias. PMID: 25092467
  3. dynamin2 orchestrates the global actin cytoskeleton PMID: 24710573
  4. Disruption of the DNM2-dependent retrograde trafficking of NHE1 contributes to cardiomyocyte apoptosis. PMID: 23837875
  5. dynamin isoforms select appropriate vesicle reuse pathways associated with specific neuronal firing patterns PMID: 23687302
  6. This study suggested that myelination is strictly dependent on Dnm2 and clathrin-mediated endocytosis function. PMID: 22451505
  7. ITSN-1s, via its SH3A domain has the unique ability to regulate dyn2 assembly-disassembly and function during endocytosis PMID: 21129155
  8. Dynamin 2 and cortactin co-localize in advancing growth cones of embryonic hippocampal neurons. PMID: 21210813
  9. Dynamin 2 is primarily tetrameric throughout the entire cell membrane. PMID: 21281565
  10. These findings support the view that endophilin-dynamin interactions play an important role in endocytosis. PMID: 21281588
  11. Formation of abnormally large and stable complexes of these dynamin mutants in vivo contributes to their role in centronuclear myopathies pathogenesis PMID: 20529869
  12. Role of the proline-rich domain of dynamin-2 and its interactions with Src homology 3 domains during endocytosis of the AT1 angiotensin receptor PMID: 11925437
  13. Localization of dynamin 2 in rat seminiferous tubules during the spermatogenic cycle. PMID: 12199526
  14. there is a distinct signaling network originating from the dopamine receptor that regulates the state of dynamin-2 phosphorylation and that promotes its location at the site of Na(+),K(+)-ATPase endocytosis PMID: 12205083
  15. The dynamins comprise a large family of mechanoenzymes known to participate in membrane modeling events. All three conventional dynamin genes (Dnm1, Dnm2, Dnm3) are expressed in mammalian brain and produce more than 27 different dynamin proteins. PMID: 12646135
  16. dynamin plays role in Rac trafficking to allow for lamellipodia formation and cell spreading PMID: 14617821
  17. Src-mediated phosphorylation of dynamin-2 is an essential requirement for scission of caveolae and the resultant transendothelial transport of albumin PMID: 15007081
  18. the first demonstration of the co-expression of all three dynamin isoforms in a non-tumor cell PMID: 15126636
  19. differential binding of SH3 domain-containing proteins to dynamin-1 and -2 may contribute to the distinct functions performed by these isoforms PMID: 15834155
  20. Data show that swapping the highly homologous GTPase domain of dynamin-2 into dynamin-1 is sufficient to confer caspase-3 activation. PMID: 16938290
  21. Dnm2 mediates fluid-phase micropinocytosis in epithelial cells. PMID: 18003703
  22. cooperative function of dynamin 2 and amphiphysin 1 in phosphatidylserine-dependent phagocytosis is strongly suggested in sertoli cells PMID: 19122684
  23. Dynamin2 GTPase and cortactin remodel actin filaments. PMID: 19605363

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Membrane, clathrin-coated pit. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein.; [Isoform 2]: Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin-coated pit.; [Isoform 4]: Cell membrane.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Tissue Specificity
Ubiquitously expressed. Brain expression is restricted to glial cells and fibroblasts. Highest levels in the testis.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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