Recombinant Rat Glutamate [NMDA] receptor subunit epsilon-4 (Grin2d), partial

Code CSB-YP733833RA
MSDS
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Source Yeast
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Code CSB-EP733833RA
MSDS
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Source E.coli
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Code CSB-EP733833RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733833RA
MSDS
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Source Baculovirus
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Code CSB-MP733833RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Grin2d
Uniprot No.
Alternative Names
Grin2d; GluN2D; Glutamate receptor ionotropic; NMDA 2D; GluN2D; Glutamate [NMDA] receptor subunit epsilon-4; N-methyl D-aspartate receptor subtype 2D; NMDAR2D; NR2D
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
Gene References into Functions
  1. Data from molecular modeling suggest that GluN2D-containing NMDA receptors can enter a conformation or mode that allows the pore to gate with high probability of burst of activity. PMID: 23578394
  2. NR2D expression falls significantly from postnatal day P2 to P3, then levels off until P12, and declines again until P21. PMID: 20887777
  3. Expression of NR2D mRNAs in the thalamus was increased by mustard oil application to molar tooth pulp. This was prevented by lidocaine & reversed by MK-801. Naloxone antagonized this reversal. PMID: 21114966
  4. At 24 hours after pulp exposure, the expression levels of NR2D mRNA were up-regulated in the contralateral thalamus. PMID: 20171363
  5. only ERalpha, not ERbeta, could upregulate transcription from the ERE which is in the 3' untranslated region of the NR2D gene. PMID: 12372009
  6. conserved threonine residue plays a crucial role in ligand binding to NMDA NR2 receptor subunits PMID: 15107472
  7. NMDAR-2D subunit is well poised anatomically to modulate pain neurotransmission, but the expression pattern for this subunit is not altered in rats demonstrating the presence of neuropathic-like pain behavior PMID: 18585442

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family, NR2D/GRIN2D subfamily
Tissue Specificity
Expressed in brain, mainly in the subcortical region.
Database Links
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