Recombinant Rat Hemopexin (Hpx)

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Code CSB-EP010723RA
MSDS
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP010723RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Hpx.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP010723RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Hpx.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Hpx
Uniprot No.
Research Area
Others
Alternative Names
Hpx; Hemopexin
Species
Rattus norvegicus (Rat)
Source
E.coli
Expression Region
24-460aa
Target Protein Sequence
NPLPAAHETVAKGENGTKPDSDVIEHCSDAWSFDATTMDHNGTMLFFKGEFVWRGHSGIRELISERWKNPVTSVDAAFRGPDSVFLIKEDKVWVYPPEKKENGYPKLFQEEFPGIPYPPDAAVECHRGECQSEGVLFFQGNRKWFWDFATRTQKERSWPAVGNCTAALRWLERYYCFQGNKFLRFNPVTGEVPPRYPLDARDYFISCPGRGHGKLRNGTAHGNSTHPMHSRCNADPGLSALLSDHRGATYAFSGSHYWRLDSSRDGWHSWPIAHHWPQGPSAVDAAFSWDEKVYLIQGTQVYVFLTKGGNNLVSGYPKRLEKELGSPPGISLDTIDAAFSCPGSSKLYVTSGRRLWWLDLKSGAQATWAELSWPHEKVDGALCLEKSLGPYSCSSNGPNLFFIHGPNLYCYSSIDKLNAAKSLPQPQKVNSILGCSQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
52.9kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This Recombinant Rat Hemopexin is engineered specifically to cater to your research needs. The Hemopexin protein, encoded by the 'Hpx' gene in the Rat species, plays a pivotal role in heme binding and transportation, thus rendering it essential for studies related to heme metabolism.

Expressed in an E.coli system, this protein replicates the full length of the mature protein, ranging from amino acid 24 to 460. We have enhanced this protein with an N-terminal 6xHis tag for facilitating purification and tracking processes. A purity level of more than 90% ascertained by SDS-PAGE substantiates the high quality of the product. It is provided in both liquid and lyophilized powder forms to support your specific experimental requirements. Our Recombinant Rat Hemopexin guarantees high reliability and precision in your research.

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Target Background

Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Gene References into Functions
  1. Data suggest that apo-hemopexin isolated from plasma exchibits low endogenous levels of tyrosine nitration in the peptide YYCFQGNQFLR in the heme-binding site of human hemopexin, which was similarly nitrated in rabbit and rat hemopexins; heme binding by hemopexin declined as tyrosine nitration proceeded in vitro. PMID: 28596380
  2. Hemopexin was identified as upregulated protein in the small intestine exposed to indomethacin. PMID: 22486875
  3. hemopexin mRNA was low or undetectable in livers of hypophysectomized rats, however, daily treatment with bovine growth hormone for 10 days restored hemopexin mRNA levels PMID: 12850285
  4. intracellular calcium and protein kinase C activation are involved in the regulation of Hpx. PMID: 12850286
Subcellular Location
Secreted.
Protein Families
Hemopexin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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