Recombinant Rat Hepatocyte nuclear factor 4-alpha (Hnf4a)

Code CSB-YP010594RA
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Source Yeast
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Code CSB-EP010594RA
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Source E.coli
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Code CSB-EP010594RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010594RA
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Source Baculovirus
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Code CSB-MP010594RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hnf4a
Uniprot No.
Alternative Names
Hnf4a; Hnf-4; Hnf4; Nr2a1; Tcf14Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
Species
Rattus norvegicus (Rat)
Expression Region
1-474
Target Protein Sequence
MRLSKTLADM DMADYSAALD PAYTTLEFEN VQVLTMGNDT SPSEGANLNS SNSLGVSALC AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR QCVVDKDKRN QCRYCRLKKC FRAGMKKEAV QNERDRISTR RSSYEDSSLP SINALLQAEV LSQQITSPIS GINGDIRAKK IANITDVCES MKEQLLVLVE WAKYIPAFCE LLLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYACLK AIIFFDPDAK GLSDPGKIKR LRSQVQVSLE DYINDRQYDS RGRFGELLLL LPTLQSITWQ MIEQIQFIKL FGMAKIDNLL QEMLLGGSAS DAPHAHHPLH PHLMQEHMGT NVIVANTMPS HLSNGQMCEW PRPRGQAATP ETPQPSPPSG SGSESYKLLP GAITTIVKPP SAIPQPTITK QEAI
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Gene References into Functions
  1. HNF4alpha constrains proliferation, migration, and maltransformation of hepatic progenitors. PMID: 28807057
  2. Transplantation of HNF4A overexpressing immortalized human hepatocytes (IHH) resulted in better liver function and survival of rats with acute liver failure (ALF) compared with IHH. HNF4A improved hepatic differentiation of IHH. Transplantation of HNF4A-overexpressing IHH could improve the liver function and survival in a rat model of ALF. PMID: 28870599
  3. Tg737 regulates a Wnt/beta-catenin/Snail-HNF4alpha negative feedback circuit, thereby blocking EMT and the malignant transformation of liver stem cells to liver cancer stem cells. PMID: 28536011
  4. HNF4alpha-NF-kappaB feedback circuit including miR-124, miR-7, and miR-21 identified in hepatocellular carcinoma PMID: 24752868
  5. Apoc3 expression is regulated by HNF4a and COUP-TFII but not by retinoic acid in rat hepatocytes. PMID: 24234421
  6. Dietary medium chain triglycerides prevent alcohol-induced hepatic lipid accumulation by preserving HNF4alpha levels in liver. PMID: 23126616
  7. Methyl donor deficiency impairs fatty acid oxidation through PGC-1alpha hypomethylation and decreased ER-alpha, ERR-alpha, and HNF-4alpha in the rat liver. PMID: 22521344
  8. Data suggest that environmentally induced epigenetic changes at the Hnf4a enhancer alter its interaction with the P2 promoter, and consequently determine T2D risk. PMID: 21385945
  9. The hepatic expression and activity of PPARalpha, SREBP-1, and hepatocyte nuclear factor-4alpha (HNF-4alpha) were investigated in sedentary rats, were investigated. PMID: 20938723
  10. Analysis of gene expression profile induced by hepatocyte nuclear factor 4alpha in hepatoma cells using an oligonucleotide microarray PMID: 11834723
  11. Ligand specificity and conformational dependence PMID: 11940586
  12. Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes. PMID: 11950391
  13. HNF-4alpha is involved in regulating cancer cell transmigration by modulating the Fas-FasL system PMID: 12036449
  14. role in bile acid inhibition of sterol 12alpha-hydroxylase gene PMID: 12069850
  15. spermine significantly enhanced the interaction between HNF4alpha and full-length DRIP205 in an AF-2, NR-box-dependent manner. Spermine enhanced the interaction of DRIP205 with the VDR , but decreased the interaction of both HNF4alpha and VDR with GRIP1 PMID: 12089346
  16. Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
  17. These findings suggest that fatty acids are endogenous ligands for HNF4 alpha and establish a framework for understanding how HNF4 alpha activity is enhanced by ligand binding and diminished by MODY1 mutations. PMID: 12193589
  18. Data show that the N-terminal activation function (AF)-1 of the hepatocyte nuclear factor (HNF)4alpha1 isoform has significant activity, but the HNF4alpha7 isoform has no measurable AF-1, implying major functional differences between the isoforms. PMID: 12205093
  19. effects mediated by HNF4alpha isoforms in concert with three different coregulators result in promoter-specific responses PMID: 12650919
  20. HNF4a has a role in regulating glucose-6-phosphatase gene expression in cultured hepatocytes and H4IIE cells PMID: 12915406
  21. the regulatory F-domain has a role in hepatocyte nuclear factor-4alpha ligand specificity PMID: 15741159
  22. HNF-4alpha transcriptional activity is controlled by its two interrelated acyl ligands and two binding sites interphased in tandem by the thioesterase activity PMID: 15870076
  23. HNF-4alpha plays a role in the maintenance of morphologically and biochemically functional hepatocytes PMID: 16087161
  24. Prenatal dexamethasone exposure caused a permanent increase in hepatic Hnf4a mRNA, which might mediate subsequent hyperglycemia. PMID: 16570165
  25. HNF4alpha isoforms, alpha1, alpha2, alpha7, and alpha8 were present in liver stem cells, which contrasted with only alpha1 and alpha2 expression in hepatocytes. PMID: 16639723
  26. nuclear expression of HNF-4alpha and CAR is an integral part of the phenobarbital response, aimed at coordinated regulation of genes involved in drug metabolism and detoxification as well as maintenance of liver function. PMID: 16799975
  27. These observations establish for the first time that HNF-4alpha functions in vivo through a direct repeat-1 (DR-1) element in the distal FAS promoter to enhance gene transcription following refeeding of glucose to fasted rats. PMID: 16800817
  28. there is a functional link between the p38 signaling pathway, HNF-4alpha, and bile acid synthesis PMID: 17603092
  29. Upregulation of the basolateral bile acid transporters Ntcp, Oatp1, and Oatp2 in fasted rats is mediated via the HNF4alpha/PGC-1alpha pathway. PMID: 17640976
  30. Dyssregulation of HNF 4 alpha and TRPC1 may be a possible molecular rationale in diabetic nephropathy. PMID: 18184923
  31. HNF4alpha 9 was expressed in rat Ins-1 insulinoma cells. PMID: 18561282
  32. berberine could prevent fructose-induced insulin resistance in rats possibly by promoting the expression HNF-4alpha in liver PMID: 18563319
  33. High-level expression of SeP in liver is ensured by concerted action of the coactivator PGC-1alpha and the transcription factors FoxO1a and HNF-4alpha. PMID: 18972406
  34. downregulation of Tcf4 at the time of cloacal separation into rectum and urethra might be related to the development of anorectal malformations. PMID: 19387659
  35. DHEA production is regulated by PGC-1alpha, ERRalpha, and HNF4alpha PMID: 19389810
  36. cAMP and glucose signaling converge on a complex containing ChREBP, HNF4alpha, and CBP, and cAMP acts by disrupting this transcriptional complex assembled by glucose-derived signals PMID: 19406844
  37. large-for-gestational-age status affects the hepatic expression of hnf4a suggesting that further research on this issue is warranted PMID: 19435144
  38. study evidences activity of HNF4alpha in human and rat choroid plexus PMID: 19575803
  39. These findings suggest that the direct suppression of very low density lipoprotein secretion by dietary n-3 polyunsaturated fatty acids delivered to the liver in chylomicron remnants is mediated via decreased expression of HNF-4alpha. PMID: 19699314

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR2 subfamily
Tissue Specificity
Liver, kidney and intestine.
Database Links
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