Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Rat Il1rap protein at 2 μg/mL can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC50 is 3.180-4.082 ng/mL.

Target Names

Il1rap

Uniprot No.

Q63621

Alternative Names

Interleukin-1 receptor accessory protein; EC:3.2.2.6; IL-1 receptor accessory protein; IL-1RAcP; Il1rap

Species

Rattus norvegicus (Rat)

Source

Mammalian cell

Expression Region

21-367aa

Target Protein Sequence

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMRLPVHRLYIEQGIHNITCPNVDGYFPSSVKPSVTWYKGCTEIVNFHNVQPKGMNLSFFIPLVSNNGNYTCVVTYLENGRLFHLTRTMTVKVVGSPKDAVPPHIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEIWWTIDGKKPDDVPVDITIIESVSYSSTEDETRTQILSIKKVTPEDLKRNYVCHARNAEGEAEQAAKVKQKVIPPRYTVELACGFGAT

Mol. Weight

41.4 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-1 receptor accessory protein forms an essential co-receptor complex with IL-1R1 to transduce pro-inflammatory signals, making it a critical target for dissecting cytokine signaling mechanisms and screening anti-inflammatory therapeutics. This mammalian-expressed construct spanning residues 21–367 captures the extracellular domain responsible for receptor assembly and ligand recognition, with native post-translational modifications that preserve conformational epitopes required for antibody interaction studies. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 3.180–4.082 ng/mL, confirming that the protein maintains its native structure and supports applications including therapeutic antibody epitope mapping, competitive inhibition screening of IL-1 pathway antagonists, and affinity characterization by surface plasmon resonance or biolayer interferometry. The combination of >95% purity and <1.0 EU/μg endotoxin satisfies the quality criteria typical for quantitative binding assays and cell-based functional studies where low endotoxin interference is essential.

Target Background

Function

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD. May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.

Gene References into Functions

Activity of Il-1 in parturition is regulated by progesterone via Il1R1/Il1R2/Il-1RAcp pathway. PMID: 27440742

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Interleukin-1 receptor family

Tissue Specificity

Highly expressed in hypothalamus, in the dentate gyrus of hippocampus, cerebral cortex, cerebellum, liver and lung.

Database Links

KEGG: rno:25466

UniGene: Rn.10511

Code	CSB-MP733954RA
Abbreviation	Recombinant Rat Il1rap protein, partial (Active)
MSDS	MSDS Datasheet
Size	$116
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Rat Il1rap protein at 2 μg/ml can bind Anti-Il1RAP recombinant antibody (CSB-RA878844MA2HU). The EC₅₀ is 3.180-4.082 ng/mL. Biological Activity Assay
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rat Interleukin-1 receptor accessory protein (Il1rap), partial (Active)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage