Recombinant Rat Mothers against decapentaplegic homolog 4(Smad4)

Code CSB-YP021789RA
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Source Yeast
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Code CSB-EP021789RA
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Source E.coli
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Code CSB-EP021789RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021789RA
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Source Baculovirus
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Code CSB-MP021789RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Smad4
Uniprot No. O70437
Alternative Names Smad4; Madh4; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; SMAD family member 4; SMAD 4; Smad4
Species Rattus norvegicus (Rat)
Expression Region 1-552
Target Protein Sequence MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPPSMLVKD EYVHDFEGQP SLPTEGHSIQ TIQHPPSNRA STETYSAPAL LAPSESNATS TTNFPNIPVA STSQPASILA GSHSEGLLQI ASGPQPGQQQ NGFTAQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 (By similarity).
Gene References into Functions
  1. This study identified a novel role of Smad4 SUMOylation and TPM2 in learning and memory formation. These results suggest that patients with skeletal myopathies who carry the TPM2E122K mutation may also have deficits in learning and memory functions. PMID: 29183317
  2. The results demonstrate that miR124 upregulation inhibits the growth of C6 glioma cells by targeting Smad4 directly. PMID: 28791348
  3. Smad4-dependent TGF-beta signaling in smooth muscle cells protects against aortic aneurysm formation and dissection. PMID: 26699655
  4. These results suggest that high glucose may activate TGF- beta/Smad signaling through sumoylation of Samd4 by SUMO2/3 in mesangial cells. PMID: 24971350
  5. miR-146a is overexpressed in an experimentally induced osteoarthritis model, accompanied by upregulation of VEGF and downregulation of Smad4 in vivo PMID: 22507670
  6. Bioinformatics analyses predict that Smad4 is the potential target of miR-146a. PMID: 22735812
  7. MicroRNA mimics, inhibitors and siRNA studies indicate the role of SMAD4 as inhibitory for glucose transport activities in normal physiological condition. PMID: 22506032
  8. Schisandrin B can reduce pulmonary fibrosis induced by SiO2 through inhibition of mRNA expression of TGF-beta1 and Smad4 in the lung tissue. PMID: 21941776
  9. TGF-beta1 and Smad4 may be involved in the pathogenesis of chronic nonbacterial prostatitis. PMID: 20608350
  10. The results showed that the protein of Smad4 was present in rats from 3 days of age to adulthood, and the immunoreactivity for Smad4 was exclusively localized to the cytoplasm of Leydig cells with negative nuclei in the interstitial tissue. PMID: 15344310
  11. Expression of BMP receptors Ib and II, and Smad 4 was downregulated in lungs but not kidneys of MCT-treated rats. PMID: 17347486
  12. Data show that SMAD4 triggers the transition from hypertrophy to apoptosis in ventricular cardiomyocytes, and suggest that it may trigger apoptosis induction and therefore contributes to negative remodeling and heart failure progression. PMID: 19415695

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Subcellular Location Cytoplasm, Nucleus
Protein Families Dwarfin/SMAD family
Database Links

KEGG: rno:50554

UniGene: Rn.9774

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