Recombinant Rat Neurogenin-1 (Neurog1)

Code CSB-YP015730RA
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Source Yeast
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Code CSB-EP015730RA
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Source E.coli
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Code CSB-EP015730RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015730RA
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Source Baculovirus
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Code CSB-MP015730RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Neurog1
Uniprot No.
Alternative Names
Neurog1; Neurod3; Ngn; Ngn1Neurogenin-1; NGN-1; Neurogenic basic-helix-loop-helix protein; Neurogenic differentiation factor 3; NeuroD3
Species
Rattus norvegicus (Rat)
Expression Region
1-244
Target Protein Sequence
MPAPLETCLS DLDCASSNSG SDLSSFLTDE EDCARLQPLA STSGLSVPAR RSAPTLSGAS NVPGGQDEEQ ERRRRRGRAR VRSEALLHSL RRSRRVKAND RERNRMHNLN AALDALRSVL PSFPDDTKLT KIETLRFAYN YIWALAETLR LADQGLPGGG ARERLLPPQC VPCLPGPPSP ASDTESWGSG AAASPCATVA SPLSDPSSPS ASEDFTYGPG GPLFSFPGLP KDLLHTTPCF IPYH
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.
Gene References into Functions
  1. Tumor necrosis factor-alpha converting enzyme (TACE) may play a role in modulating repulsive guidance molecule (RGM)-induced repulsive behavior of neurons by regulating neogenin expression on the cell surface. PMID: 21645559
  2. Comparison of the generic neuronal differentiation and neuron subtype specification functions of mammalian achaete-scute and atonal homologs in cultured neural progenitor cells. PMID: 11923194
  3. Ngn1 is required for Akt-mediated neuronal differentiation and neurite outgrowth PMID: 16136056
  4. Results identify ERK5 phosphorylation of Neurogenin 1 as a novel mechanism regulating neuronal fate commitment of cortical progenitors. PMID: 19365559
Subcellular Location
Nucleus.
Tissue Specificity
Expression restricted to the embryonic nervous system.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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