Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Pla2g2a

Uniprot No.

P14423

Research Area

Others

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

22-146aa

Target Protein Sequence

SLLEFGQMILFKTGKRADVSYGFYGCHCGVGGRGSPKDATDWCCVTHDCCYNRLEKRGCGTKFLTYKFSYRGGQISCSTNQDSCRKQLCQCDKAAAECFARNKKSYSLKYQFYPNKFCKGKTPSC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To express the recombinant rat phospholipase A2, membrane associated (Pla2g2a) in E. coli, the gene fragment encoding the full-length mature rat Pla2g2a protein (amino acids 22-146) and an N-terminal 6xHis-tag is cloned into a suitable expression vector. The cloning process involves ligating the gene fragment into the vector downstream of the appropriate promoter and regulatory elements. The resulting expression construct is then transformed into E. coli host cells using a transformation method. The transformed cells are selected to ensure the presence of the recombinant plasmid. Subsequently, a large-scale culture of the transformed E. coli cells is established in a growth medium under optimal conditions for protein expression. The N-terminal 6xHis-tag allows for convenient purification and detection of the recombinant Pla2g2a protein. The recombinant Pla2g2a protein can be extracted and purified from the cell lysate. The purity of the recombinant human Pla2g2a protein is assessed through SDS-PAGE analysis, reaching up to 90%. It presents as a band of 64 molecular weight on the gel.

Target Background

Function

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. Simultaneously, by compromising mitochondrial membrane integrity, promotes the release in circulation of potent damage-associated molecular pattern molecules that activate the innate immune response. Plays a stem cell regulator role in the intestinal crypt. Within intracellular compartment mediates Paneth cell differentiation and its stem cell supporting functions by inhibiting Wnt signaling pathway in intestinal stem cell (ICS). Secreted in the intestinal lumen upon inflammation, acts in an autocrine way and promotes prostaglandin E2 synthesis that stimulates Wnt signaling pathway in ICS cells and tissue regeneration. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.

Gene References into Functions

The present study was designed to determine the relationship between phenformin coupling AMP-activated protein kinase (AMPK) function and the molecular mechanism by which the sPLA2 IIA expression was modulated in vascular smooth muscle cells. PMID: 26162096
a novel inverse recruitment mechanism in which liganded TRbeta recruits corepressors to inhibit PLA2g2a expression. PMID: 23629656
Secreted PLA2-IIA regulates the permeability of microvascular endothelial cells during brain inflammation. PMID: 22788969
Data show changes in cell morphology and upregulation of ERK1/2, iNOS and sPLA-IIA expression in astrocytes and microglia on exposure to lipopolysaccharides and cytokines. PMID: 21943492
cPLA(2)alpha-mediated induction of PPAR-beta/delta is a novel intracellular signalling pathway in spontaneous and TGF-beta induced activation of hepatic stellate cells. PMID: 22093332
The expression of sPLA(2)-IIA in the dorsal horn and spinal trigeminal nucleus is consistent with previous results which showed an important role of CNS sPLA(2) in nociceptive transmission. PMID: 20153419
calcium-independent phospholipase A2beta has a role in high glucose-induced activation of RhoA, Rho kinase, and CPI-17 in cultured vascular smooth muscle cells and vascular smooth muscle hypercontractility in diabetic animals PMID: 20086008
During atherogenesis, SAA can amplify the involvement of smooth muscle cells in vascular inflammation and that this can lead to deposition of sPLA(2) and subsequent local changes in lipid homeostasis. PMID: 19850938
A calcium-independent, type IIA secretory phospholipase A2 newly isolated from liver mitochondria, most apparent in media of high ionic strength, initiates the removal of poorly functioning mitochondria by processes involving autolysis. PMID: 12056909
sPLA(2) may modulate neuronal COX-2 expression through mechanisms that differ from those of glutamate-induced COX-2 expression PMID: 12111798
analysed the activation of sPLA(2) transcription by cAMP and interleukin-1beta and showed that the 500 bp region upstream of the transcription start site of the sPLA(2) gene is implicated in activation by synergistically acting cAMP and IL-1beta PMID: 12188923
tumor necrosis factor alpha-induced secreted phospholipase A2 (sPLA2)-IIA expression is potentiated in mesangial cells by an autocrine loop involving sPLA2 and peroxisome proliferator-activated receptor alpha activation PMID: 12782627
Transcriptional activation of the group IIA secretory phospholipase A2 gene in vascular smooth muscle. PMID: 12882648
in a neonatal model, the development of an acidic surface pH can be ascribed, in part, to progressive stratum corneum acidification by two endogenous mechanisms, namely, sPLA2 and NHE1 PMID: 15009712
We investigated temporal and spatial expression of sPLA2-IIA mRNA and immunoreactivity in transient focal cerebral ischemia, demonstrating an up-regulation of the inflammatory sPLA2-IIA in reactive astrocytes in response to cerebral ischemia-reperfusion. PMID: 15255941
new autocrine and paracrine pathways activating sPLA2-IIA gene expression in rat and human vascular smooth muscle cells PMID: 15802623
12/15-LOX-dependent up-regulation of sPLA2-IIA expression may result from the interplay between accelerated MIP-2 signaling, AP-1 activation, and attenuated transforming growth factor-beta and platelet-derived growth factor signaling PMID: 15878884
the neuritogenic effect of sPLA2 is mediated by generation of LPC and subsequent activation of G2A PMID: 15927955
These results indicate that phospholipase A(2)-IIA is coexpressed with SNAP-25 in mature taste receptor cells that possess exocytotic machinery. PMID: 16385482
cytosolic and group IIA secreted phospholipases A(2) work together to liberate arachidonate from phospholipids in response to cytokines PMID: 16603549
HMGB1 slightly activated the sPLA2-IIA, COX-2, and mPGES-1 genes but dramatically stimulated these genes in VSMCs that had been incubated with the proinflammatory cytokine IL-1beta PMID: 16807371
It was shown that exposure of cultured astrocytes to oxygen glucose deprivation (0.5-24h) causes an increase in cPLA(2) and sPLA(2) expression and activity. PMID: 17462919
a novel regulatory role of iPLA(2)gamma in stimulus-coupled sPLA(2)-IIA expression. PMID: 17475622
On Chr 5, Pla2g2a is subject to a syntenic control while expression of the Tp53 (Chr 10) and Pmai1/Noxa (Chr 18) genes appears to be controlled by several mammary cancer resistance QTLs. PMID: 19052818
Simvastatin may reduce the process of atherosclerosis by decreasing the expression level of sPLA2 IIa in myocardium and aorta. PMID: 19237014
sPLA(2)-IIA may be an important mediator of oligodendrocyte death and a novel target for therapeutic intervention following spinal cord injury PMID: 19306380
Reactive oxygen species from NADPH oxidase iare involved in cytokine induction of sPLA2-IIA in astrocytes. Botanical antioxidants may be protective agents for inhibition of inflammatory responses in these cells. PMID: 19375465
sPLA2-derived oxidative products contribute to significant neurovascular damage, and treatment with sPLA2 inhibitor DEDA ameliorates secondary injury by reducing exacerbations from lipoxidative stress. PMID: 19678934

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Subcellular Location

Secreted. Cell membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein.

Protein Families

Phospholipase A2 family

Database Links

KEGG: rno:29692

STRING: 10116.ENSRNOP00000022827

UniGene: Rn.11346

Code	CSB-EP321246RAa0
Abbreviation	Recombinant Rat Pla2g2a protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Phospholipase A2, membrane associated (Pla2g2a)

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