Product Details

Purity

>85% (SDS-PAGE)

Target Names

Atp2b2

Uniprot No.

P11506

Alternative Names

Atp2b2Plasma membrane calcium-transporting ATPase 2; PMCA2; EC 7.2.2.10; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems. Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment. Has fast activation and Ca(2+) clearance rate suited to control fast neuronal Ca(2+) dynamics. At parallel fiber to Purkinje neuron synapse, mediates presynaptic Ca(2+) efflux in response to climbing fiber-induced Ca(2+) rise. Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning. Plays an essential role in hearing and balance. In cochlear hair cells, shuttles Ca(2+) ions from stereocilia to the endolymph and dissipates Ca(2+) transients generated by the opening of the mechanoelectrical transduction channels. Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia. In non-excitable cells, regulates Ca(2+) signaling through spatial control of Ca(2+) ions extrusion and dissipation of Ca(2+) transients generated by store-operated channels. In lactating mammary gland, allows for the high content of Ca(2+) ions in the milk.

Gene References into Functions

higher occupancy of Calm2 and Calm3 promoters by NFATc2 and increased expression of these genes in response to NFATc2 silencing were demonstrated in PMCA2 and PMCA3-reduced lines. PMID: 28153703
The results with PMCA2- or PMCA3-downregulated lines provide an evidence of a novel role of PMCA isoforms in regulation of bioenergetic pathways. PMID: 25276815
Plasma membrane PMCA2 and PMCA3 regulate cellular pH homeostasis in differentiating adrenal chromaffin cell line in a manner dependent on cytosolic Ca2+ elevations. PMID: 25014339
GlyT2 activity is pre-synaptically controlled by physical and functional association with plasma membrane PMCA2, PMCA3, and NCX1. PMID: 25315779
Results demonstrate a significant impairment of cell survival under conditions of PMCA2 suppression. Overexpression of PMCA2 rendered the neurons significantly resistant to complex I inhibition. PMID: 24269647
The distribution of PMCA2 at different cochlear locations during development was quantified. PMID: 22672315
Results suggest that PMCA2 and PMCA3 function is rather related to regulation of GABA synthesis and degradation than supplying cells with metabolites, which can be potentially energetic source. PMID: 21798237
Spiral ganglion cells from newborn rats strongly expresses PMCA2. PMID: 20398509
Data link the reduction in PMCA2 expression with perturbations in CRMP1 expression and death of spinal cord neurons, representing an additional mechanism underlying AMPA/kainate receptor-mediated excitotoxicity with relevance to neurodegeneration in EAE. PMID: 20489728
A novel function for PMCAs as modulators of Ca(2+) signaling at the synapse is suggested. PMID: 20678993
PMCA2w/a is hair-bundle isoform of PMCA in rat hair cells, and splice variant 2w targets PMCA2 to bundles. The 2w sequence is first targeting signal identified for a hair-bundle membrane protein PMID: 16763025
PMCA2 molecules maintain transient interactions with other components of stereocilia, and mobile pool of PMCA2 mediates exchange between stereocilia and removal and delivery sites at periphery of apical cell surface. PMID: 16763047
A Leu-Ile motif in 'b'-tail splice variants promotes PMCA1b and PMCA2b basolateral sorting in hair cells. PMID: 16803870
fast PMCA2a-mediated clearance of [Ca2+]i from presynaptic excitatory terminals regulated excitatory synaptic transmission within hippocampal CA3 PMID: 17170045
PMCA2 is targeted to distinct compartments within the plasma membrane. In the molecular layer, this isoform was at highest levels within postsynaptic profiles of the cerebellar cortex. PMID: 17183553
The evidence suggests that PMCA contributes to re-establish resting Ca(2+) levels in the cilia following olfactory responses. PMID: 17970729
overexpression of PMCA2 leads, in insulin-producing cells, to an imbalance between ATP generation and consumption. PMID: 18643776
Results suggest that the fast Ca(2+) activation kinetics and high V(max) of PMCA2a make this pump especially suited for rapid clearance of presynaptic Ca(2+) in fast-spiking inhibitory nerve terminals, which face severe transient calcium loads. PMID: 19025983
Results indicate that hormones affected Ca(2+) transport activity via PMCA2, and that this effect depended on both PMCA isoform composition and steroid structure. PMID: 19120150
Reduced expression of PMCA2a during homeostatic plasticity provides a mechanism to remodel pre-synaptic Ca2+ dynamics as a flexible way to alter glutamate release. PMID: 19410650
Changes in islet plasma membrane calcium ATPase activity and Atp2b2 protein isoform expression induced by insulin reistance are reported. PMID: 19653992

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, synapse. Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIB subfamily

Tissue Specificity

Isoforms containing segment B are found in brain, uterus, liver and kidney and in low levels in other tissues. Isoforms containing segment W are found in kidney, uterus, and pancreas. Isoforms containing segment Y are found in pancreas and in low levels i

Database Links

KEGG: rno:24215

UniGene: Rn.90982

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Recombinant Rat Plasma membrane calcium-transporting ATPase 2 (Atp2b2), partial

Product Details

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Target Background

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