Recombinant Rat Presenilin-1(Psen1),partial

Code CSB-YP018846RA
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Source Yeast
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Code CSB-EP018846RA
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Source E.coli
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Code CSB-EP018846RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018846RA
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Source Baculovirus
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Code CSB-MP018846RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Psen1
Uniprot No. P97887
Alternative Names Psen1; Psnl1; Presenilin-1; PS-1; EC 3.4.23.-; Protein S182) [Cleaved into: Presenilin-1 NTF subunit; Presenilin-1 CTF subunit; Presenilin-1 CTF12; PS1-CTF12)]
Species Rattus norvegicus (Rat)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity. Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (By similarity). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity).
Gene References into Functions
  1. Cultured hippocampal neurons expressing mutant PS1 had attenuated CCE that was associated with destabilized dendritic spines, which were rescued by either gamma-secretase inhibition or overexpression of STIM1. PMID: 27601731
  2. PS1 expression is triggered by glutamate through p38alpha, contributing to the excitotoxic stimulus of EW PMID: 27278235
  3. Quantitative reverse-transcriptase PCR assay demonstrated that the expression of APP, beta- and gamma-secretase, and PSEN1 that contribute to Abeta peptide generation was dysregulated during 30 days in postischemic hippocampal CA1 area PMID: 26401782
  4. Vitamin E may depress the production of Abeta by regulating the expression of PS-1 in the hippocampus of female senile rats. PMID: 21179780
  5. Presenilin/gamma-secretase regulates neurexin processing at synapses PMID: 21559374
  6. PS1, APP and Abeta double staining with cell-type specific markers showed colocalization of PS1 with axons in the white matter of the lesioned side. These findings suggest that RIP signaling occurs following rat spinal cord injury. PMID: 19932144
  7. protects against neuronal apoptosis caused by its interacting protein PAG. PMID: 11895366
  8. Presenilin-1 is located in rat mitochondria. PMID: 12099705
  9. Presenilin 1 appears to be developmentally regulated in the retina, and the pattern is consistent with a role in retinal lamination and pattern formation. PMID: 12401445
  10. experiments establish amyloid precursor protein, nicastrin, and presenilin-1 as resident lysosomal membrane proteins and indicate that gamma-secretase is a lysosomal protease PMID: 12736250
  11. Nicastrin, presenilin, APH-1, and PEN-2 form active gamma-secretase complexes in mitochondria PMID: 15456764
  12. amyloid precursor protein and presenilin 1 packaging into coat protein complex II vesicles is normally uncoupled PMID: 15623526
  13. The amount of presenilin-1 in the nucleus and membrane fraction was more abundantly expressed in the denervated muscle fibers. In contrast, no significant difference in the nicastrin protein level was observed between normal and denervated muscle fibers. PMID: 16595164
  14. Together, [3H]-L-685,458 is an excellent radiotracer for mapping active gamma-secretase complex, and may serve as a useful tool for studying the enzyme in vivo and in vitro. PMID: 17512915
  15. The C-terminal fragment of PS-1, necessary for gamma-secretase activity, was significantly reduced 12h following passive avoidance conditioning. PMID: 17543552
  16. presenilin 1 C-terminal fragment contents in kidney, liver, brain and heart were 17.0, 6.6, 6.4 and 0.2 fmol/mg protein, respectively PMID: 19318429
  17. After induction of apoptosis with staurosporine (Sts), a dramatic increase in the level of PS1-CTF in the medium was observed. PMID: 11570818

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Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein, Golgi apparatus membrane, Multi-pass membrane protein, Cytoplasmic granule, Cell membrane
Protein Families Peptidase A22A family
Tissue Specificity Detected in embryonic and adult brain.
Database Links

KEGG: rno:29192

STRING: 10116.ENSRNOP00000012495

UniGene: Rn.44440

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