Recombinant Rat Sodium-dependent dopamine transporter (Slc6a3), partial

1. Results show that Gbetagamma activation regulates DAT activity by increasing dopamine (DA) efflux, and suggests that Gbetagamma promotes an efflux-willing state of the transporter and that the activation of a GPCR can lead to a Gbetagamma-dependent DA efflux. Furthermore, the cellular responses that occur following activation of a Galphaq-coupled receptor are the result of multiple signaling pathways mediated by Galpha... PMID: 28894302
2. a new genetic rat model with a loss-of-function point mutation in the dopamine transporter (DAT) gene (Slc6a3_N157K) was used to systematically study the research domain criteria matrix. PMID: 28167616
3. Study compared showing a clear-cut profile of either gambling proneness or aversion, found a selective down-regulation of i) serotonin transporter in prefrontal cortex, ii) tyrosine hydroxylase in ventral striatum, iii) dopamine transporter in lymphocytes was evidenced in "gambler" vs "non-gambler" rats. PMID: 27789384
4. Together these results support the hypothesis that sigma1R agonists promote dissociation of sigma1R multimers into monomers, which then interact with DAT to stabilize an outward-facing DAT conformation and enhance cocaine binding. PMID: 28495886
5. The present study suggested that amphetamine-induced circling behavior indicated striatal dopaminergic alterations and that dopamine transporter (DAT) and dopamine D2 receptor binding could be key markers for predicting motor dysfunction after mild focal ischemia. PMID: 26911894
6. Increased expression of DAT is associated with visceral obesity PMID: 26707058
7. Data show that Fischer 344 rats displayed greater tyrosine hydroxylase and dopamine transporter levels in the frontal cortex compared to the Lewis rats. PMID: 26658810
8. Study indicated that enhanced infralimbic DAT function is a consequence of adolescent social stress exposure PMID: 26056032
9. Ctr9, formerly identified as a nuclei protein, interacts with the dopamine transporter in the cytoplasmic compartment. PMID: 26048990
10. Data suggest that expression of Dat1 and Vmat2 (vesicular monoamine transporter 2) in dopaminergic neurons in striatum can be down-regulated by dietary factors (here, ingestion of acrylamide, a food contaminant created by heating starchy foods). PMID: 25943760
11. Comparative modeling and small molecule docking indicated that the tropane pharmacophore of RTI 82 was positioned in the central DA active site with an orientation that juxtaposed the aryliodoazide group for cross-linking to rat DAT Phe-319. PMID: 25179220
12. Data indicate that internalized dopamine transporter (DAT) colocalized with GFP-Rab11 and suggest that Rab11 plays an important role in the recycling of DAT following AMPH-induced DAT down-regulation. PMID: 23612789
13. Dopamine transporter distribution in the cytoplasm and synaptosomes of substantia nigra in later life is linked to previous environmental toxin exposure. PMID: 23567316
14. In corpus striatum slices, basal activity of Akt2 regulates DAT cell surface expression. PMID: 22778840
15. Dopamine transporter loss in Parkinson's disease PMID: 23300642
16. Both dopamine transporter and VMAT2 are inhibited by HIV-1 Tat protein in corpus striatum synaptosomes. PMID: 22570010
17. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding PMID: 23161550
18. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. PMID: 22722938
19. Maternal separation altered the dopaminergic system by decreasing surface expression of dopamine transporter (DAT) and/or the affinity of DAT for dopamine, in the striatum of spontaneously hypertensive rats. PMID: 22133315
20. Temporal overexpression of LRRK2(G2019S) in adult rats impaired dopamine reuptake by dopamine transporter and thus enhanced locomotor activity. PMID: 21698001
21. These results indicate a role for the active dopamine transporter in mediating the suppression of TH expression in midbrain dopaminergic neurons by antipsychotic drugs. PMID: 21396352
22. Data suggest that axonal dopamine transporters (DAT) mainly shuttle between the plasma membrane and recycling endosomes, whereas in the somatodendritic region of neurons DAT traffics through all conventional endosomal pathways. PMID: 20816972
23. These results reveal a novel role for CPE in the regulation of DAT trafficking and DAT-mediated dopamine uptake. PMID: 19419578
24. S-palmitoylation as a major regulator of DAT properties that could significantly impact acute and long term dopamine transport capacity. PMID: 21118819
25. Data support the hypothesis that the dopamine transporter (DAT) adopts an outward facing conformation in a cholesterol-rich membrane environment, suggesting a novel modulatory role of the surrounding membrane lipid milieu on DAT function. PMID: 20688912
26. Data indicate that selected derivatives showed high in vitro potency and selectivity at SERT, NET, and DAT. PMID: 20170186
27. These results strongly suggest that DAT glycosylation is involved in the differential vulnerability of midbrain dopamine cells in Parkinson's disease. PMID: 19766189
28. Data show that the wild-type dopamine transporter displays apparent affinities for substrate efflux more than 300-fold lower than those for uptake. PMID: 12480180
29. dopamine membrane transporter (DAT) protein density and mRNA expression is upregulated in restricted-fed adult male rats PMID: 12521926
30. dopamine transporters are a direct substrate for protein phosphatase 1 in vitro and suggest that PP1 is a major DAT phosphatase in rat striatum. PMID: 12573538
31. dopamine transporter constitutively internalizes and recycles in a protein kinase C-regulated manner in stably transfected PC12 cell lines PMID: 12682063
32. Observed differences in density and distribution of DAT1 sites in Wistar Kyoto rats may lead to altered modulation of synaptic dopamine levels in cell body and mesolimbic regions, contributing to the depression-like behaviors reported in this rat strain. PMID: 14499307
33. tyrosine hydroxylase and the dopamine transporter are differentially distributed in the medial- versus the lateral ventral pallidum. PMID: 14681933
34. the carboxyl terminus regulates dopamine transporter export from endoplasmic reticulum PMID: 15128747
35. The decrease in DAT cell surface expression in the mPFC may be responsible for decreased DAT function in the mPFC of stimulated compared with deprived rats; there is plasticity in the regulatory mechanisms mediating DAT trafficking and function. PMID: 15935059
36. This expression profile of the dopamine transporter suggests that dopamine may be taken up not only in the synapses but also extrasynaptically by dopamine transporter, diffusely distributed in both plexiform layers. PMID: 16708013
37. Cocaine binding near TM6 may overlap the dopamine translocation pathway and function to inhibit TM6 structural rearrangements necessary for transport PMID: 17255098
38. Lactotroph cells expressing TH coexpressed dopamine transporter and cleaved caspase-3 postlactation. PMID: 17363452
39. Data show that valproate treatment significantly increases dopamine transporter gene expression in a Sp transcription factor-dependent manner. PMID: 17439486
40. This is the first evidence showing that DAT can be activated by the firing activity in nigrostriatal cells in a direct way and without the participation of pre-synaptic DA receptors PMID: 17561841
41. The pharmacological effect of nicotine to increase DAT function and cell surface expression was apparent, such that rats with inherently lower DAT function show a greater sensitivity to the neurochemical effect of nicotine. PMID: 17651428
42. Relationship between conformational changes in the dopamine transporter and cocaine-like subjective effects of uptake inhibitors is reported. PMID: 17978168
43. chronic cocaine self-administration followed by abstinence leads to persisting alterations in normal DAT1 trafficking and catalytic regulatory cascades in the CPu and NAcc in a brain region-specific manner. PMID: 18198344
44. These results directly establish the proximity of transmembrane domains 1 and 6 in DAT and suggest that the mechanism of transport inhibition by cocaine involves close interactions with multiple regions of the substrate permeation pathway. PMID: 18216182
45. Coinjection of lentiviruses expressing DAT and gamma-synuclein - leading to overexpression of both proteins in the NAc - resulted in a strong increase in cocaine-induced rat locomotor activity suggesting a synergetic role of both proteins. PMID: 18588534
46. It has been found that GBR12909 is capable of inducing so-called "slow isomerization step" upon binding to DAT, probably consisting of a conformational change in the transporter protein. PMID: 18845198
47. These data suggest that neuronal activity can regulate dopamine transporter function and abundance via calcium/CaM kinase II signaling. PMID: 19046383
48. Dopamine transporter over-expression in rats' nucleus accumbens leads to impulsive and risk prone phenotype. PMID: 19135135

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KEGG: rno:24898

STRING: 10116.ENSRNOP00000047272

UniGene: Rn.10093