Recombinant Rat Sulfotransferase 1A1 (Sult1a1)

Code CSB-YP022933RA
MSDS
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Source Yeast
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Code CSB-EP022933RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022933RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022933RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sult1a1
Uniprot No.
Alternative Names
Sult1a1; St1a1; Sulfotransferase 1A1; ST1A1; EC 2.8.2.1; Aryl sulfotransferase; Aryl sulfotransferase IV; ASTIV; Minoxidil sulfotransferase; Mx-ST; PST-1; Phenol sulfotransferase; Sulfokinase; Tyrosine-ester sulfotransferase
Species
Rattus norvegicus (Rat)
Expression Region
1-291
Target Protein Sequence
MEFSRPPLVH VKGIPLIKYF AETIGPLQNF TAWPDDLLIS TYPKSGTTWM SEILDMIYQG GKLEKCGRAP IYARVPFLEF KCPGVPSGLE TLEETPAPRL LKTHLPLSLL PQSLLDQKVK VIYIARNAKD VVVSYYNFYN MAKLHPDPGT WDSFLENFMD GEVSYGSWYQ HVKEWWELRH THPVLYLFYE DIKENPKREI KKILEFLGRS LPEETVDSIV HHTSFKKMKE NCMTNYTTIP TEIMDHNVSP FMRKGTTGDW KNTFTVAQNE RFDAHYAKTM TDCDFKFRCE L
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of a wide variety of acceptor molecules bearing a hydroxyl or an amine groupe. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Displays broad substrate specificity for small phenolic compounds. Plays an important roles in the sulfonation of endogenous molecules such as steroid hormones and 3,3'-diiodothyronin. Mediates the sulfate conjugation of a variety of xenobiotics, including the drugs acetaminophen and minoxidil. Mediates also the metabolic activation of carcinogenic N-hydroxyarylamines leading to highly reactive intermediates capable of forming DNA adducts, potentially resulting in mutagenesis.
Gene References into Functions
  1. SULT1A1 is responsible for bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol. PMID: 25370010
  2. Higher SULT1A1 levels were observed in rats as well as in humans exposed to high altitude, when compared to sea-level controls PMID: 26022216
  3. Results suggest that the physiological functions of SULT1A1 in different brain regions can be affected by methamphetamine. PMID: 23026138
  4. Data suggest that Sult1a1 and Sult2a1 can be induced in small intestinal mucosa by dietary factors (here, by various levels of caffeine); induction is gender specific and organ specific. Consumption of caffeine may enhance xenobiotic detoxification. PMID: 21721019
  5. mechanistic studies on enzyme activity of purified recombinant Sult1a1 and Sult1a1 in viable liver slices after treatment with the same oxidants [1,1'-azobis(N,N-dimethylformamide); tert-butylhydroperoxide] and the same substrate (7-hydroxycoumarin) PMID: 22041107
  6. Stp1 is important for appropriate regulation of Stk1 function, hemolysin activity, autolysis, and GBS virulence PMID: 22081606
  7. there is a possible Cys modification mechanism for stress regulation of SULT1A1 PMID: 15351727

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Subcellular Location
Cytoplasm.
Protein Families
Sulfotransferase 1 family
Tissue Specificity
Liver, kidney, heart and colon.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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