Name: Recombinant Rat Thyrotropin receptor (Tshr), partial
Brand: CUSABIO
SKU: 025131RA1

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Tshr

Uniprot No.

P21463

Alternative Names

Tshr; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

Could you produce this item with N-terminal 6xHis tag and HEK293 cell ?
Thank you for your help.

Thanks for your inquiry.
Recombinant Rat Thyrotropin receptor(Tshr),partial
CSB-YP025131RA1 >> Yeast
CSB-EP025131RA1 >> E.coli
CSB-BP025131RA1 >> Baculovirus
CSB-MP025131RA1 >> Mammalian cell
Expression Region: 22-413aa; The extracellular domain.
Tag information: Tag type will be determined during the manufacturing process.
We can try your desired tag preferentially if you have, but as we can't 100% guaranteed, if it's failed, we will provide the protein with other tag.
The success rate of expressing the protein with your desired tag is 95.25%.
Target protein sequence: (The whole complete sequence will include tag sequene, target protein sequence and any linker sequence)

RGCTSPPCECHQEDDFRVTCKELHQIPSLPPSTQTLKLIETHLKTIPSLAFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRIFPDLTKIYSTDVFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTAIDKDAFGGVYSGPTLLDVSSTSVTALPSKGLEHLKELIAKNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSIRNLRQRKSVNVMRGPVYQEYEEGLGDNHVGYKQNSKFQEGPSNSHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTVCGDNEDMVCTPKSDEFNPCEDIMG

Yes, we can produce this protein with N-terminal 6xHis tag and HEK293 cell, pls remark your requirement when you place the order.

We are interested in your following product: Recombinant Rat Thyrotropin receptor(Tshr),partial
1. Could you please let us know the expression region and tag information of this product?
2. Please let us know the formulation and preservative information of this product.
Thank you for your support. Looking forward to hearing from you.

Thanks for your inquiry. Pls check the information as follows:
Recombinant Rat Thyrotropin receptor(Tshr),partial
CSB-YP025131RA1 >> Yeast
CSB-EP025131RA1 >> E.coli
CSB-BP025131RA1 >> Baculovirus
CSB-MP025131RA1 >> Mammalian cell
Expression Region: 22-413aa; The extracellular domain.
Tag information: Tag type will be determined during the manufacturing process.
We can try your desired tag preferentially if you have, but as we can't 100% guaranteed, if it's failed, we will provide the protein with other tag.
The success rate of expressing the protein with your desired tag is 95.25%.
Target protein sequence: (The whole complete sequence will include tag sequene, target protein sequence and any linker sequence)

The buffer formulation for this protein is Tris-based buffer, 50% glycerol.
Storage:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Target Background

Function

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.

Gene References into Functions

Data suggest that functional thyroid-stimulating hormone receptor is present in the caudal brainstem and hypothalamic nuclei of relevance for feeding control as a possibly uncleaved holoreceptor, and highlights a hindbrain component to central thyroid-stimulating hormone inhibition of food intake. PMID: 27059392
TSH signal transduction in cultured tanycytes signals via G alphas to increase cAMP via an alternative G protein to increase phosphorylation of ERK1/2. PMID: 25878058
Results show that Tshr mRNA levels were up-regulated during differentiation of brown adipocytes. During the differentiation of brown adipocytes PMID: 25662278
Higher TSH level in the BN strain appears to reflect an adjustment of the feedback loop to reduced thyrocyte sensitivity to TSH secondary to reduced TSHR expression. PMID: 17640981
TSHR is present and functional in hepatocytes. PMID: 19187127
Data indicate that the presence of thyrostimulin in the ovary suggests that it may have a primitive function in reproduction when it activates ovarian TSHR. PMID: 19955180
Developmental profiles of TSH, sex steroids, and their receptors in the thyroid and their relevance to thyroid growth in immature rats PMID: 11755178
Identical amino acid residues in homologous receptors can exhibit distinct functions. Role of ionic strength (Na+) on signaling differs in the three receptors. PMID: 12588044
mutation effects vary with the ambient iodide supply and could be masked in regions of high iodine intake PMID: 15691889

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily

Database Links

KEGG: rno:25360

STRING: 10116.ENSRNOP00000005671

UniGene: Rn.87913

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Code	CSB-YP025131RA1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP025131RA1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP025131RA1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP025131RA1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP025131RA1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rat Thyrotropin receptor (Tshr), partial

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