TSHR, a glycoprotein receptor part of the LGR subfamily, also belongs to the A-class G protein coupled receptors (GPCRs) superfamily. It is primarily found in thyroid tissue but is also present in nerve tissue, immune cells, eye muscles, bones, adipocytes, red blood cells, ovaries, and the liver. TSHR functions by detecting thyroid stimulating hormone (TSH) signals from pituitary cells, initiating a coupled signaling pathway that promotes the expression of downstream effector genes, stimulates the production of thyroid hormones (T4 and T3), and supports thyroid growth and function. Abnormal activation or dysregulation of TSHR is linked to several diseases; for instance, in Graves' disease, patients produce autoantibodies that activate TSHR, resulting in hyperthyroidism and potentially Graves' ophthalmopathy, characterized by eye muscle swelling. Dysfunction in TSHR can also impair thyroid hormone production, leading to hypothyroidism, as seen in Hashimoto's disease where antibodies inhibit TSHR. Changes in TSHR expression in specific thyroid nodules and cancers can serve as valuable diagnostic and prognostic markers.
Recombinant Human Thyrotropin receptor (TSHR)-VLPs (Active) (CSB-MP025131HU)
Validated Data
CSB-MP025131HU is detected by Mouse anti-6*His monoclonal antibody.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TSHR at 10 μg/ml can bind Anti-TSHR recombinant antibody (CSB-RA025131MA1HU). The EC50 is 30.81-36.12 ng/mL.The VLPs (CSB-MP3838) is negative control.
The purity of VLPs was greater than 90% as determined by SEC-HPLC
TSHR Recombinant Monoclonal Antibody (CSB-RA025131MA1HU)
Validated Data
The Binding Activity of Human TSHR with Anti-TSHR Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TSHR (CSB-MP025131HU) at 10 μg/mL can bind Anti-TSHR recombinant antibody. The EC50 is 30.81-36.12 ng/mL.The VLPs (CSB-MP3838) is negative control.
Untransfected HEK293T cells (green line) and transfected Human TSHR HEK293T stable cells (red line) were stained with anti-TSHR antibody (CSB-RA025131MA1HU) (2µg/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
The purity of TSHR was greater than 95% as determined by SEC-HPLC
The following TSHR reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TSHR Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA11189D0Rb | TSHR Antibody, Biotin conjugated | Human | ELISA |
CSB-PA11189C0Rb | TSHR Antibody, FITC conjugated | Human | |
CSB-PA11189B0Rb | TSHR Antibody, HRP conjugated | Human | ELISA |
CSB-PA11189A0Rb | TSHR Antibody | Human | ELISA, IF |
CSB-PA025131GA01HU | TSHR Antibody | Human,Mouse | ELISA,WB |
CSB-PA973863 | TSHR Antibody | Human | ELISA,IHC |
CSB-PA820027 | TSHR Antibody | Human | ELISA,IHC |
CSB-RA025131MA1HU | TSHR Recombinant Monoclonal Antibody | Human | ELISA |
TSHR Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP025131MO CSB-EP025131MO CSB-EP025131MO-B |
Recombinant Mouse Thyrotropin receptor (Tshr), partial | Yeast E.coli In Vivo Biotinylation in E.coli |
CSB-BP025131MO | Recombinant Mouse Thyrotropin receptor (Tshr), partial | Baculovirus |
CSB-MP025131MO | Recombinant Mouse Thyrotropin receptor (Tshr), partial | Mammalian cell |
TSHR Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF631684BO | Recombinant Bovine Thyrotropin receptor (TSHR) | in vitro E.coli expression system |
CSB-YP631684BO1 CSB-EP631684BO1 CSB-BP631684BO1 CSB-MP631684BO1 CSB-EP631684BO1-B |
Recombinant Bovine Thyrotropin receptor (TSHR), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Felis catus (Cat) (Felis silvestris catus)
Code | Product Name | Source |
---|---|---|
CSB-CF858993CA | Recombinant Cat Thyrotropin receptor (TSHR) | in vitro E.coli expression system |
CSB-YP858993CA1 CSB-EP858993CA1 CSB-BP858993CA1 CSB-MP858993CA1 CSB-EP858993CA1-B |
Recombinant Cat Thyrotropin receptor (TSHR), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-CF025131RA | Recombinant Rat Thyrotropin receptor (Tshr) | in vitro E.coli expression system |
CSB-YP025131RA1 CSB-EP025131RA1 CSB-BP025131RA1 CSB-MP025131RA1 CSB-EP025131RA1-B |
Recombinant Rat Thyrotropin receptor (Tshr), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Ovis aries (Sheep)
Code | Product Name | Source |
---|---|---|
CSB-CF025131SH | Recombinant Sheep Thyrotropin receptor (TSHR) | in vitro E.coli expression system |
CSB-YP025131SH1 CSB-EP025131SH1 CSB-BP025131SH1 CSB-MP025131SH1 CSB-EP025131SH1-B |
Recombinant Sheep Thyrotropin receptor (TSHR), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-CF025131DO | Recombinant Dog Thyrotropin receptor (TSHR) | in vitro E.coli expression system |
CSB-YP025131DO1 CSB-EP025131DO1 CSB-BP025131DO1 CSB-MP025131DO1 CSB-EP025131DO1-B |
Recombinant Dog Thyrotropin receptor (TSHR), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP819360PI CSB-EP819360PI CSB-BP819360PI CSB-MP819360PI CSB-EP819360PI-B |
Recombinant Pig Thyrotropin receptor (TSHR), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
TSHR Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-BP025131HU1 | Recombinant Human Thyrotropin receptor (TSHR), partial | Baculovirus |
CSB-MP025131HU | Recombinant Human Thyrotropin receptor (TSHR)-VLPs (Active) | Mammalian cell |