Name: Recombinant Rat Tyrosine-protein kinase Mer (Mertk), partial
Brand: CUSABIO
SKU: CSB-MP013706RA

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Mertk

Uniprot No.

P57097

Research Area

Neuroscience

Alternative Names

Mertk; MerTyrosine-protein kinase Mer; EC 2.7.10.1; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK

Species

Rattus norvegicus (Rat)

Source

Mammalian cell

Expression Region

19-497aa

Target Protein Sequence

GGTAEKEEEIKPDQPFSGPLPGSLPADHRPFFAPHSSGDQLSPSQTGRSHPAHTATPQMTSAASNLLPPVAFKNTIGRIVLSEHKSVKFNCSINIPNVYQETAGISWWKDGKELLGAHHSITQFYPDEEGVSIIALFSITSVQRSDNGSYICKMKVNDREVVSDPIYVEVQGLPYFTKQPESVNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNENPERSPSVLTVAGLTETAVFSCEAHNDKGLTVSKGVQINIKVIPSPPTEVHILNSTAHSILVSWVPGFDGYSPLQNCSIQVKEADQLSNGSVMVFNTSASPHLYEVQQLQALANYSVTVSCRNEIGWSAVSPWILASTTEGAPAVAPLNITVFLNESSNNLEIRWTKPPIKRQDGELVGYRISHVWESAGTSKELSEEVSQNGSWAQVPVQMHNATCTVRIAVITKGGIGPFSEPVDVAIPEHSRVDYAPSSTPAPGNTESM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

55.5

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

We would like to order CSB-MP013706RA – 0.05mg.
Before moving forward, could it be confirmed for the tag to be 6xHis (customers first choice) or GST?
Thank you in advance for your assistance.

Sorry, as this is a semi-customized protein that we haven't expressed before, so the tag type can't be confirmed in advance.
However, we have rich experience and mature technology, etc. We can preferentially try to express the 6xHis (your first choice) or GST Tag,
The success rate should be greater than 95.25%, but could you please let us know if you need the tag located at the N-terminus or C-terminus?
Please remark your desired tag when placing the order, we can try to express your desired tag preferentially.

Thank you for the below information.
Question: If the tag 6xHis (customer's first choice) or GST Tag is not successful, will you replace the tag information to one of the following expected tags?
MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.

If the tag 6xHis (customer's first choice) or GST Tag is not successful, we would like to check if you need any C-terminal tag,
Since the expected tag for this protein is N-terminal 10xHis-tagged and C-terminal Myc-tagged, if you don't want C-terminal Tag,
we can 100% guarantee to remove the C-terminal successfully, and the final tag would be N-terminal 10xHis-tagged.
If you want to keep the C-terminal Tag, then the final tag would be N-terminal 10xHis-tagged and C-terminal Myc-tagged.

Target Background

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Gene References into Functions

Mertk protein expression in the Bone marrow mesenchymal stem cells was similar to that in the retinal pigment epithelial cells in the primary passage. PMID: 28210098
study concludes that activated microglia release Gal-3 and a neuraminidase that desialylates microglial and PC12 surfaces, enabling Gal-3 binding to PC12 (neuron-like) cells and their phagocytosis via Mer tyrosine kinase (MerTK). PMID: 28500071
This study demonstrated that in Mertk mutation mice regulated the Expression pattern of Ccr2 and Cx3cr1. PMID: 26458944
A novel role of MERTK signaling in membrane trafficking in the Retinal Pigment Eepithelium that is likely to subserve mechanisms of phagosome formation. PMID: 26283020
A deletion in the Mer tyrosine kinase (MerTK) receptor in Royal College of Surgeons rat that abolishes internalization of photoreceptor outer segments by retinal pigment epithelial cells. PMID: 20238011
Mertk triggers uptake of photoreceptor outer segments during phagocytosis by cultured retinal pigment epithelial cells. PMID: 11861639
Key involvement of mer in outer segment phsgocytosis has been clearly proven. PMID: 15180281

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Involvement in disease

Defects in Mertk are the cause of retinal dystrophy (rdy) in the royal college of surgeons (RCS) rats.

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily

Database Links

KEGG: rno:65037

STRING: 10116.ENSRNOP00000023419

UniGene: Rn.48789

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