Recombinant Rat Vesicular inhibitory amino acid transporter (Slc32a1), partial

Code CSB-YP021578RA
MSDS
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Source Yeast
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Code CSB-EP021578RA
MSDS
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Source E.coli
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Code CSB-EP021578RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021578RA
MSDS
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Source Baculovirus
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Code CSB-MP021578RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc32a1
Uniprot No.
Alternative Names
Slc32a1; Vgat; ViaatVesicular inhibitory amino acid transporter; GABA and glycine transporter; Solute carrier family 32 member 1; Vesicular GABA transporter; rGVAT
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the uptake of GABA and glycine into the synaptic vesicles.
Gene References into Functions
  1. cortical axon terminals co-expressing VGLUT1 and VGAT can evoke simultaneously monosynaptic glutamate and gamma-aminobutyric acid responses. PMID: 25749864
  2. Data show that VIAAT is a vesicular Cl(-) transporter that co-transports Cl(-) with GABA or glycine in a membrane-potential-dependent manner, and that Cl(-) plays an essential role in vesicular storage of GABA and glycine. PMID: 19843525
  3. VIAAT is expressed in rat islets and is more abundant in the mantle PMID: 12031963
  4. functional neuronal VIAAT is present in glucagon-containing secretory granules in pancreatic alpha-cells PMID: 12882924
  5. In mature neocortex neuronal cultures, VGLUT1 and VIAAT exhibit bidirectional and opposite regulation by prolonged activity changes. PMID: 16079394
  6. As a whole, the results provide original evidence that vGAT expression is altered in the 6-hydroxydopamine model of Parkinson's disease. PMID: 17218060
  7. Concomitantly with the loss of some subpopulations of VGAT-containing neurons, there was an up-regulation of VGAT synthesis in all remaining GABA neurons as early as 1 week after pilocarpine injection. PMID: 17503488
  8. Glycine is released from synaptic vesicles when VIAAT is coexpressed with the neural transporter GlyT2. PMID: 17554001
  9. VGLUT1 is sorted diurnally to the plasma membrane to modulate glutamate transmission during a day/night cycle, whereas VGAT expression is not oscillating but is increased in the presence of a light/dark cycle. PMID: 19103593
  10. GABAergic synapses in cortical axon terminals express both vesicular transporters VGAT and VGLUT1. PMID: 19627441

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Subcellular Location
Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Protein Families
Amino acid/polyamine transporter 2 family
Tissue Specificity
Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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