Recombinant Rotavirus A Outer capsid glycoprotein VP7

Code CSB-MP420195RIU
Size Pls inquire other sizes
Source Mammalian cell
Code CSB-EP420195RIU-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

Product Details

Purity >85% (SDS-PAGE)
Uniprot No. A8D8S8
Species Rotavirus A (strain RVA/Cow/Canada/C486/1977/G6P6[1]) (RV-A)
Expression Region 34-309
Target Protein Sequence QNYGVNL PITGSMDTAY ANSTQSEPFL TSTLCLYYPV EASNEIADTE WKDTLSQLFL TKGWPTGSVY LKEYADIAAF SVEPQLYCDY NLVLMKYDST QELDMSELAD LILNEWLCNP MDITLYYYQQ TDEANKWISM GSSCTVKVCP LNTQTLGIGC LITNPDTFET VATTEKLVIT DVVDGVSHKL NVTTATCTIR NCKKLGPKEN VAVIQVGGAN ILDITADPTT TPQTERMMAI IWKKWWQVVY PVVDYVNQII QTMSKRSRSL NSSAFYYRV
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).
Subcellular Location Virion, Host endoplasmic reticulum lumen
Protein Families Rotavirus VP7 family

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