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Recombinant Saccharomyces cerevisiae Bud site selection protein RAX2 (RAX2), partial

Recombinant Saccharomyces cerevisiae Bud site selection protein RAX2 (RAX2), partial

Code	CSB-YP623624SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP623624SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP623624SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP623624SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP623624SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RAX2

Uniprot No.

Q12465

Alternative Names

RAX2; YLR084C; L2389; L9449.12; Bud site selection protein RAX2; Revert to axial protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for the maintenance of the bipolar budding pattern. Involved in selecting bud sites at both the distal and proximal poles of daughter cells as well as near previously used division sites on mother cells.

Gene References into Functions

These observations suggest that the interaction of Rax2p with Bud8p and Bud9p may contribute to the translocation of bipolar landmarks to the correct sites. PMID: 20678480
Rax1p and Rax2p interact closely with each other and with Bud8p and Bud9p in the establishment and/or maintenance of the cortical landmarks for bipolar budding PMID: 15356260

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Bud neck. Bud tip. Note=Before cytokinesis, RAX2 concentrates as a ring at the mother-bud neck and to the tip of the bud. The RAX2 ring splits at cytokinesis, endowing each progeny cell with a RAX2 ring and additional RAX2 localization at the distal bud pole of the newborn daughter cell. The rings persist at the cell cortex for several generations, giving rise to cells decorated by multiple rings.

Database Links

KEGG: sce:YLR084C

STRING: 4932.YLR084C

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