Recombinant Saccharomyces cerevisiae Mediator of replication checkpoint protein 1 (MRC1), partial

1. An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. PMID: 29371596
2. Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1. PMID: 27956499
3. Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts. PMID: 18321795
4. Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1. PMID: 23165348
5. Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress. PMID: 22298423
6. Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53. PMID: 21840863
7. cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase PMID: 20219942
8. Data show that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled. PMID: 15755447
9. Mrc1 functions constitutively to promote normal replication fork progression. PMID: 16137624
10. Tof1p-Csm3p function differently from MRC1 and their complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae PMID: 16418273
11. Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to proliferating cell nuclear antigen (PCNA) independently of checkpoint signaling. PMID: 16724109
12. Mrc1 may negatively regulate Cdc45 and MCM helicase to render stalled forks capable of resuming replication. PMID: 16849602
13. Study reports that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence. PMID: 17323081
14. analyzed sister-chromatid cohesion in double mutants of mrc1Delta, tof1Delta, and csm3Delta and identified additive cohesion defects that indicated the existence of at least two pathways that contribute to sister-chromatid cohesion PMID: 17483413
15. Mrc1 and Tof1 control the normal progression of DNA replication forks in distinct ways. PMID: 17652453
16. In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). PMID: 18851837
17. Data show that the replication checkpoint suppresses the formation of Rad52 foci in an Mrc1-dependent manner and prevents homologous recombination (HR) at chromosome breaks induced by the HO endonuclease. PMID: 19322196
18. Rad53 is activated by Mec1 through adaptor protein Mrc1 PMID: 19457865
19. Mec1-dependent, Rad53-independent phosphorylation of Mrc1 is required to establish a positive feedback loop that stabilizes Mec1 and the replisome at stalled forks. PMID: 19515819
20. An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. PMID: 19620285
21. Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks PMID: 19819872

Show More

Hide All

KEGG: sce:YCL061C

STRING: 4932.YCL061C