Product Details

Uniprot No.

P25588

Target Names

MRC1

Alternative Names

Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) MRC1 YCL061C YCL61C/YCL60C

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MRC1 protein (711-851aa)

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To generate polyclonal antibodies against MRC1, a rabbit is systematically immunized with recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MRC1 protein (711-851aa). After reaching an optimal antibody titer, the rabbit is bled, and antibodies are extracted from the serum and then undergo affinity chromatography purification. The functionality of the resultant MRC1 antibody is then assessed in both ELISA and WB applications, demonstrating specificity towards Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MRC1 protein.

The Saccharomyces cerevisiae MRC1 is involved in the regulation of DNA replication and the cellular response to DNA damage, thus maintaining chromosomal stability, preventing the accumulation of genetic mutations, and ensuring accurate transmission of genetic information during cell division in Saccharomyces cerevisiae. Essential for stabilizing and maintaining the integrity of the replication fork during DNA replication, MRC1 prevents replication fork collapse and facilitates smooth replication machinery progression. Additionally, MRC1 also participates in signaling pathways that mediate DNA repair and contribute to genomic integrity.

Target Background

Function

Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.

Gene References into Functions

An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. PMID: 29371596
Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1. PMID: 27956499
Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts. PMID: 18321795
Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1. PMID: 23165348
Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress. PMID: 22298423
Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53. PMID: 21840863
cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase PMID: 20219942
Data show that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled. PMID: 15755447
Mrc1 functions constitutively to promote normal replication fork progression. PMID: 16137624
Tof1p-Csm3p function differently from MRC1 and their complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae PMID: 16418273
Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to proliferating cell nuclear antigen (PCNA) independently of checkpoint signaling. PMID: 16724109
Mrc1 may negatively regulate Cdc45 and MCM helicase to render stalled forks capable of resuming replication. PMID: 16849602
Study reports that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence. PMID: 17323081
analyzed sister-chromatid cohesion in double mutants of mrc1Delta, tof1Delta, and csm3Delta and identified additive cohesion defects that indicated the existence of at least two pathways that contribute to sister-chromatid cohesion PMID: 17483413
Mrc1 and Tof1 control the normal progression of DNA replication forks in distinct ways. PMID: 17652453
In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). PMID: 18851837
Data show that the replication checkpoint suppresses the formation of Rad52 foci in an Mrc1-dependent manner and prevents homologous recombination (HR) at chromosome breaks induced by the HO endonuclease. PMID: 19322196
Rad53 is activated by Mec1 through adaptor protein Mrc1 PMID: 19457865
Mec1-dependent, Rad53-independent phosphorylation of Mrc1 is required to establish a positive feedback loop that stabilizes Mec1 and the replisome at stalled forks. PMID: 19515819
An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. PMID: 19620285
Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks PMID: 19819872

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Subcellular Location

Nucleus. Note=Associated with chromatin during S phase.

Database Links

KEGG: sce:YCL061C

STRING: 4932.YCL061C

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Code	CSB-PA338680XA01SVG
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: MRC1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
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MRC1 Antibody

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