Recombinant Saccharomyces cerevisiae Polyphosphatase (PPX1)

E.coli-expressed Recombinant Saccharomyces cerevisiae Exopolyphosphatase (PPX1) is a full-length protein carrying an N-terminal 6xHis-tag. It contains 397 amino acids and has a calculated molecular mass of 49.1 kDa. Its purity was determined by SDS-PAGE and reached up to 90%. The purified PPX1 migrated on the SDS–PAGE at a molecular weight of about 50 kDa. It was also identified and quantified using LC-MS/MS. This recombinant PPX1 protein may be used to immunize animals to produce specific antibodies or in the studies of PPX1-participating inorganic polyphosphate hydrolysis. In-stock PPX1 proteins are available.

Exopolyphosphatase PPX1 is a phosphatase enzyme that catalyzes the hydrolysis of inorganic polyphosphate (polyP) and contributes to the maintenance of the polyP dynamic balance in the cells. The polyP is believed to exert a regulatory role in the transition to bacterial dormancy. Seema M. Thayil etc. demonstrated that PPX1 is required for M. tuberculosis (Mtb) growth and persistence in necrotic lung lesions, which may suggest a way to shorten latent tuberculosis (TB) treatment by targeting “persister” bacillia through the repression of a small molecule inhibitor to PPX1. In addition to degrading polyP, PPX1 has been implicated directly in bacterial virulence.