Recombinant Sheep Endothelin-1 (EDN1)

Code CSB-YP858990SH
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Source Yeast
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Code CSB-EP858990SH
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Source E.coli
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Code CSB-EP858990SH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP858990SH
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Source Baculovirus
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Code CSB-MP858990SH
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EDN1
Uniprot No.
Alternative Names
EDN1Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
Species
Ovis aries (Sheep)
Expression Region
53-73
Target Protein Sequence
CSCSSLMD KECVYFCHLD IIW
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions
  1. These results support the hypothesis that chronic hypoxia elicits sustained changes in fetal cerebrovascular reactivity to ET-1 through pathways dependent upon PKC, CaMKII, and p38 that cause increased ET-1-mediated contractility, decreased ET-1-mediated smooth muscle hypertrophy, and a depressed ability of ET-1 to promote contractile dedifferentiation. PMID: 28566491
  2. Endothelin receptor A, but not endothelin receptor B, mediates antisteroidogenic actions of endothelin 1 (EDN1) on prostaglandin (PGF2alpha)-induced luteolysis(endothelin 1). PMID: 18077801
  3. Study shows that ET-1 stimulates protein kinase Cd (PKCd) activity, which in turn attenuates eNOS transcription and NOS signaling through the activation of STAT3. PMID: 19754268
  4. Endothelin-1 has a role in reactive oxygen species production by pulmonary artery endothelial cells, leading to pulmonary artery endothelial cell dysfunction in congenital diaphragmatic hernia PMID: 25580737
  5. ET-1 decreases PPARgamma signaling and contributes to pulmonary artery endothelial cell dysfunction and impaired angiogenesis in persistent pulmonary hypertension of the newborn. PMID: 24337925
  6. Endothelin-1 impairs angiogenesis in vitro through Rho-kinase activation after chronic intrauterine pulmonary hypertension in fetal sheep. PMID: 23202724
  7. ET-1 stimulates ciliary beat frequency via interaction with endothelin A receptors; inhibition of both COX and NOS inhibited the effect of ET-1 suggesting a role for both enzymes in the ET-1 response downstream from the ETA-receptor PMID: 21664477

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Subcellular Location
Secreted.
Protein Families
Endothelin/sarafotoxin family
Database Links

KEGG: oas:443498

UniGene: Oar.807

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