Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
hlgB; SA2209; Gamma-hemolysin component B; H-gamma-1; H-gamma-I
Species
Staphylococcus aureus (strain N315)
Expression Region
26-325aa
Target Protein Sequence
AEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Staphylococcus aureus (strain N315) hlgB contains amino acids 26-325. This hlgB protein is expected to have a theoretical molecular weight of 38.1 kDa. This hlgB protein is produced using e.coli expression system. The N-terminal 6xHis tag was smoothly integrated into the coding gene of hlgB, which enables a simple process of detecting and purifying the hlgB recombinant protein in the following steps.
The Staphylococcus aureus gamma-hemolysin component B (hlgB) is a subunit of the bicomponent gamma-hemolysin toxin, which also includes HlgA. Gamma-hemolysin is known for its hemolytic activity, which involves the lysis of red blood cells. The hlgB in conjunction with HlgA, forms pores in the target cell membrane, leading to cell lysis. This cytolytic activity is implicated in the pathogenicity of S. aureus, contributing to tissue damage and evasion of the host immune response. Understanding the molecular mechanisms and regulation of gamma-hemolysin, including the role of HlgB, is crucial for developing strategies to combat S. aureus infections. Additionally, the study of this toxin provides insights into the intricate interactions between bacterial pathogens and host cells.