Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
psaA; papA; Manganese ABC transporter substrate-binding lipoprotein; Pneumococcal surface adhesin A
Species
Streptococcus pneumoniae
Expression Region
21-310aa
Target Protein Sequence
CSKGTSSKSSSDKLKVVTTNSILADITKNIAGDKIELHSIVPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVKNANKVENKDYFAASDGVEVIYLEGQNQAGKEDPHAWLNLENGIIYAKNIAKQLIAKDPKNKDFYEKNLAAYTEKLSKLDQEAKQAFNNIPAEKKMIVTSEGCFKYFSKAYGVPSAYIWEINTEVEGTPEQIKTLLEKLRQTKVPSLFVESSVDERPMKTVSKDSNIPIFAKIFTDSIAKEGEEGDSYYSMMKWNLEKIAEGLNK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer，50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Streptococcus pneumoniae psaA contains amino acids 21-310. The theoretical molecular weight of the psaA protein is 39.9 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of psaA, which enables a simple process of detecting and purifying the psaA recombinant protein in the following steps.
The Streptococcus pneumoniae manganese ABC transporter substrate-binding lipoprotein, encoded by the psaA gene, is a crucial component of the ABC (ATP-binding cassette) transport system responsible for the acquisition of manganese ions in the bacterium Streptococcus pneumoniae. PsaA acts as the substrate-binding component of the ABC transporter, recognizing, binding, and transporting manganese across the bacterial cell membrane. The lipoprotein anchor in PsaA allows it to associate with the bacterial cell membrane. The efficient acquisition of manganese is vital for the survival and virulence of Streptococcus pneumoniae, particularly in the host environment where nutrient availability is a critical factor.
