Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

psaA

Uniprot No.

P42363

Research Area

Others

Alternative Names

psaA; papA; Manganese ABC transporter substrate-binding lipoprotein; Pneumococcal surface adhesin A

Species

Streptococcus pneumoniae

Source

E.coli

Expression Region

21-310aa

Target Protein Sequence

CSKGTSSKSSSDKLKVVTTNSILADITKNIAGDKIELHSIVPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVKNANKVENKDYFAASDGVEVIYLEGQNQAGKEDPHAWLNLENGIIYAKNIAKQLIAKDPKNKDFYEKNLAAYTEKLSKLDQEAKQAFNNIPAEKKMIVTSEGCFKYFSKAYGVPSAYIWEINTEVEGTPEQIKTLLEKLRQTKVPSLFVESSVDERPMKTVSKDSNIPIFAKIFTDSIAKEGEEGDSYYSMMKWNLEKIAEGLNK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Streptococcus pneumoniae psaA contains amino acids 21-310. The theoretical molecular weight of the psaA protein is 39.9 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of psaA, which enables a simple process of detecting and purifying the psaA recombinant protein in the following steps.

The Streptococcus pneumoniae manganese ABC transporter substrate-binding lipoprotein, encoded by the psaA gene, is a crucial component of the ABC (ATP-binding cassette) transport system responsible for the acquisition of manganese ions in the bacterium Streptococcus pneumoniae. PsaA acts as the substrate-binding component of the ABC transporter, recognizing, binding, and transporting manganese across the bacterial cell membrane. The lipoprotein anchor in PsaA allows it to associate with the bacterial cell membrane. The efficient acquisition of manganese is vital for the survival and virulence of Streptococcus pneumoniae, particularly in the host environment where nutrient availability is a critical factor.

Target Background

Function

Part of an ABC transporter complex involved in manganese import.

Subcellular Location

Cell membrane; Lipid-anchor.

Protein Families

Bacterial solute-binding protein 9 family, Lipoprotein receptor antigen (Lrai) subfamily

Code	CSB-EP337054FMW
Abbreviation	Recombinant Streptococcus pneumoniae psaA protein
MSDS	MSDS Datasheet
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP337054FMW could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae psaA. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP337054FMW could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae psaA.
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Recombinant Streptococcus pneumoniae Manganese ABC transporter substrate-binding lipoprotein (psaA)

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