Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ply

Uniprot No.

Q04IN8

Research Area

others

Alternative Names

ply; SPD_1726; Pneumolysin; Thiol-activated cytolysin

Species

Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)

Source

E.coli

Expression Region

2-471aa

Target Protein Sequence

ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

72.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The LC-MS/MS Analysis-validated E.coli-expressed Recombinant Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (ply) protein is the full-length of mature protein with an N-terminal 10xHis-SUMO-tag and a C-terminal Myc-tag. The expression region corresponds to 2-471aa of Streptococcus pneumoniae serotype 2 ply protein. This recombinant ply protein has a molecular weight ranging from 66.2 kDa to 92 kDa and purity greater than 90% as determined by SDS-PAGE.

Pneumolysin (ply) is a toxic protein antigenically related to thiol-activated, cholesterol-binding cytolysins. High levels of ply can lyse all cells with cholesterol-containing membranes. Ply participates in the pathogenesis of lethal pneumococcal pneumonia probably due to its pro-apoptotic and pro-inflammatory activity.

Target Background

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein.

Protein Families

Thiol-activated cytolysin family

Database Links

KEGG: spd:SPD_1726

STRING: 373153.SPD_1726

Code	CSB-EP312102FNF
Abbreviation	Recombinant Streptococcus pneumoniae serotype 2 ply protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP312102FNF could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) ply. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP312102FNF could indicate that this peptide derived from E.coli-expressed Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) ply.
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Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply)

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