Recombinant Sulfolobus acidocaldarius DNA double-strand break repair protein Mre11 (mre11)

Code CSB-YP854171FOZ
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Source Yeast
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Code CSB-EP854171FOZ
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Source E.coli
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Code CSB-EP854171FOZ-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP854171FOZ
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Source Baculovirus
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Code CSB-MP854171FOZ
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mre11
Uniprot No.
Alternative Names
mre11; Saci_0052; DNA double-strand break repair protein Mre11; EC 3.1.-.-
Species
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Expression Region
1-382
Target Protein Sequence
MQLLHISDTH LGKRQYNLES REKDVYDTFT QLIDIAINEH VKAVIHTGDL FDVNNPPNRA KLHAIKELKR LKDHNIPFIC IAGDHDSPKR KEEIYPQRIL EEFNLIKILQ KIDNRVKLEN VEVYGISHIS NVSVNDLKEQ LSKVKPETRK SILMLHQGIR TYLPYQGAWQ IELSDLPKGF SLYAVGHLHS RRKDYLDGGA LIEIAGSPDI MREEEIEDYQ KSKKGATLID MSGDLPSINY INVNIRDQLV LDINVDKIEQ SIESVIQKLK ENVKNDKKPI LHIELEGTVP IRKDVLMTKL QALRDYVEHY RIYKNNIVSI KEENLKTKLK TTYSSLNDII ADYLKGIGYT DEETKIIIDI INEEDEKKVE ELLKKFAGVE DK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity. Recruited immediately to chromosomal DNA after gamma irradiation, and remains DNA bound in the course of DNA repair.
Protein Families
MRE11/RAD32 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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