Recombinant Xenopus laevis Casein kinase I isoform delta (csnk1d)

Code CSB-YP688559XBE
MSDS
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Source Yeast
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Code CSB-EP688559XBE
MSDS
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Source E.coli
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Code CSB-EP688559XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP688559XBE
MSDS
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Source Baculovirus
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Code CSB-MP688559XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
csnk1d
Uniprot No.
Alternative Names
csnk1d; Casein kinase I isoform delta; CKI-delta; CKId; EC 2.7.11.1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-415
Target Protein Sequence
MELRVGNRYR LGRKIGSGSF GDIYLGTDIA ASEEVAIKLE CVKTKHPQLH IESKIYKMMQ GGVGIPTIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFAT YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGASRA AEDAERERRE REERLRHTRN PAVRGLPSTA SGRLRGTQEV TPSTPLTPTS HTANTSPRPV SGMERERKVS MRLHRGAPVN VSSSDLTSRQ DTSRMSTSQI PSRVTSSGLP STVHR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Isoform 1 can phosphorylate a large number of proteins. Isoform 2 has no kinase activity. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating per1 and per2, which may lead to their degradation. Participates in wnt signaling.
Gene References into Functions
  1. The results demonstrate a high degree of evolutionary conservation of fly and vertebrate CKIdelta and of the functions affected by their period-shortening mutations. PMID: 18957703
Subcellular Location
Cytoplasm. Nucleus. Note=Isoform 1 is found in the cytoplasm and the nucleus; isoform 2 is found in the cytoplasm.
Protein Families
Protein kinase superfamily
Tissue Specificity
Detected in retina photoreceptor cells.
Database Links

KEGG: xla:380361

UniGene: Xl.1636

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