Recombinant Xenopus laevis Hepatocyte nuclear factor 4-alpha (hnf4a)

Code CSB-YP835521XBE
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Source Yeast
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Code CSB-EP835521XBE
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Source E.coli
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Code CSB-EP835521XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835521XBE
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Source Baculovirus
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Code CSB-MP835521XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hnf4a
Uniprot No.
Alternative Names
hnf4a; hnf4; nr2a1; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-464
Target Protein Sequence
MRLSKALIDM DMADYTEALD PAYTTLEFEN MQVLSIGTDT STSDVTSLSA SNSIGINSLC AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR QCVVDKDKRN QCRYCRLKKC FRAGMKKEAV QNERDRISTR RSSYEDSSLP SINVLIQAEV LSQQITSSVG VLNTDIRGKK IACIIDVCDS MKQQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMMFK DILLLGNDRL IPRNCPELEV GRVAVRILDE LVLPFQELQI DDNEYACLKA IIFFDPDAKG LSDPTKIKRM RYQVQVSLED YINDRQYDSR GRFGELLLLL PTLQSITWQM IEQIQFVKLF GMAKIDNLLQ EMLLGGSANE ASHTHHHLHP HLVQDHLATN VIVANNTLPS QLHNGQMSTP ETPQPSPPAG SGAEQYKIVH GTIASINKQP TSIPQSTITK QEAM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional regulator; binds and activates the promoter for the HNF1-alpha gene. Potential initiator of a transcriptional cascade within a subset of cells committed to a specific developmental program. Could be a determinant for asymmetry in early development. May play a role in the regulation of the circadian clock.
Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR2 subfamily
Tissue Specificity
Expressed in liver and kidney.
Database Links

KEGG: xla:379762

UniGene: Xl.1217

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