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Recombinant Xenopus laevis Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (pkmyt1), partial

Recombinant Xenopus laevis Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (pkmyt1), partial

Code	CSB-YP849597XBE
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP849597XBE
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP849597XBE-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP849597XBE
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP849597XBE
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

pkmyt1

Uniprot No.

Q91618

Alternative Names

pkmyt1; myt1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; EC 2.7.11.1; Myt1 kinase

Species

Xenopus laevis (African clawed frog)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Gene References into Functions

Data show that CDK1 and cyclin B synergize during oocyte maturation to inhibit Myt1 ensuring meiotic progression. PMID: 20362450
Study propose a model in which the appearance of active MPF complexes following increased Cyclin B synthesis causes Myt1 inhibition, upstream of the MPF/Cdc25 amplification loop. PMID: 21795279
Autophosphorylation of Myt1 is a novel inhibitory mechanism that adds another layer of complexity to the phosphorylation-dependent mechanism of Myt1 regulation. PMID: 16481744
XRINGO-activated CDKs can phosphorylate specific residues in the regulatory domain of Myt1. PMID: 18951089

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily

Database Links

KEGG: xla:379626

UniGene: Xl.15673

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