Recombinant Xenopus laevis Protein Wnt-3a(wnt3a)

Code CSB-YP026136XBE
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Source Yeast
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Code CSB-EP026136XBE
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Source E.coli
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Code CSB-EP026136XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026136XBE
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Source Baculovirus
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Code CSB-MP026136XBE
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wnt3a
Uniprot No. P31285
Alternative Names wnt3a; Protein Wnt-3a; XWnt-3a
Species Xenopus laevis (African clawed frog)
Expression Region 19-352
Target Protein Sequence TY PIWWSLAVGQ QYSSLGTQPI PCGTIPGLVA KQMRFCRNYM EIMPSVAEGV KIGIQECQHQ FRGRRWNCTT VNDNLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GSATICGCDT HHKGPPGEGW KWGGCSEDMD FGSMVSREFA DARENRPDAR SAMNRHNNEA GRTSILDHRH LKCKCHGLSG SCEVKTCWWS QPDFRVIGDY LKDKYDSASE MVVEKHRESR GWVETLRPKY TFFKPPIERD LIYYESSPNF CEPNPETGSF GTRDRECNVT SHGIDGCDLL CCGRGQNTRT EKRKEKCHCI FHWCCYVSCQ ECMRVYDVHT CK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube.
Gene References into Functions
  1. Sulf1 does not affect Wnt3a-mediated activation of canonical Wnt signaling. PMID: 25681501
  2. Wnt3a protein alone is sufficient to rescue the severe loss of inner ear structures resulting from dorsal but not ventral half ablations. PMID: 24500889
  3. hindbrain-repressive Wnt3a/Meis3/Tsh1 circuit promotes neuronal differentiation and coordinates tissue maturation PMID: 22399680
  4. Wnt3a thus acts downstream of FAK to balance anterior-posterior cell fate specification in the developing neural plate PMID: 21551070
  5. Data suggest a new model for neural anteroposterior patterning, in which Wnt3a from the paraxial mesoderm induces posterior cell fates via direct activation of a crucial transcription factor in the overlying neural plate. PMID: 20356957
  6. Wnt3A secretion from tectal cells along with ephrin-B1 signaling are specifically responsible for enhanced neural responses in the developing optic tectum. PMID: 20346764

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Subcellular Location Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families Wnt family
Tissue Specificity At neurula in anterior neural fold; at tailbud in dorsal midline of midbrain.
Database Links

KEGG: xla:378679

UniGene: Xl.880

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