Recombinant Xenopus laevis Transcription factor GATA-4 (gata4)

Code CSB-YP835518XBE
MSDS
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Source Yeast
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Code CSB-EP835518XBE
MSDS
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Source E.coli
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Code CSB-EP835518XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835518XBE
MSDS
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Source Baculovirus
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Code CSB-MP835518XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gata4
Uniprot No.
Alternative Names
gata4Transcription factor GATA-4; GATA-binding factor 4; xGATA-4
Species
Xenopus laevis (African clawed frog)
Expression Region
1-392
Target Protein Sequence
MYQSIAMATN HGPSGYEGTG SFMHSATAAT SPVYVPTTRV SSMIHSLPYL QTSGSSQQGS PVSGHNMWAQ AGVESSAYNP GTSHPPVSPR FTFSSSPPIT APSSREVSYS SPLGISANGR EQYSRGLGAT YASPYPAYMS PDMGAAWTAS PFDSSMLHNL QNRAVTSRHP NIEFFDDFSE GRECVNCGAM STPLWRRDGT GHYLCNACGL YHKMNGINRP LIKPQRRLSA SRRVGLSCAN CHTTTTTLWR RNAEGEPVCN ACGLYMKLHG VPRPLAMKKE GIQTRKRKPK NLSKSKTLTG QSGSDSLTPS TSSTNSMGEE MRPIKIEPGL SPPYDHSNSI SQASALSTIT SHGSSYYPMP SLKLSPQNHH STFNPSPQAN SKHDSWNNLV LA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3'. Associated with cardiac specification and can regulate cardiac-specific transcription during embryogenesis. Activates the expression of cardiac MHC-alpha in vivo.
Gene References into Functions
  1. The data demonstrate that KLF13 is an important component of the transcription network required for heart development and suggest that KLF13 is a GATA-4 modifier PMID: 17053787
Subcellular Location
Nucleus.
Tissue Specificity
Expressed at high levels in heart, small intestine, stomach, ovary, and liver. Found at much lower levels in lung, spleen, pancreas and skin.
Database Links

KEGG: xla:399302

UniGene: Xl.1061

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