Recombinant Xenopus tropicalis Homeobox protein OTX2(otx2)

Code CSB-YP630728XBF
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Source Yeast
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Code CSB-EP630728XBF
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Source E.coli
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Code CSB-EP630728XBF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630728XBF
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Source Baculovirus
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Code CSB-MP630728XBF
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names otx2
Uniprot No. Q28FN6
Alternative Names otx2; TGas114a06.1; Homeobox protein OTX2; Orthodenticle 2
Species Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region 1-289
Target Protein Sequence MMSYLKQPPY AVNGLSLTTS GMDLLHPSVG YPATPRKQRR ERTTFTRAQL DVLEALFAKT RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQQ QNGGQNKVRP SKKKPSPARE VSSESGTSGQ FSPPCSTSVP VISSSTAPVS IWSPASISPL SDPLSTSSSC MQRSYPMTYT QASGYSQGYA GSTSYFGGMD CGSYLTPMHH QLSGAGATLS PMGTNAVTSH LNQSPAALSS QAYGASSLGF NSTADCLDYK DQTASWKLNF NADCLDYKDQ TSSWKFQVL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function May play a central role in the initial events of axis formation and in particular in specifying anterior head regions and their spatial relationship with trunk structures. Activates the head organizer gene cer1 by acting synergistically with siamois and mix-A/mix.1 through the 5'-TAATCT-3' element of the cer1 promoter. Also binds as a complex with lhx1/lim1 and ldb1 to the gsc promoter to stimulate expression (By similarity).
Gene References into Functions
  1. Otx2 upregulate or downregulate a large battery of target genes in the head organizer. PMID: 25005894
  2. The obligate integration of inputs of a field-selector (Otx2) and localized signaling (Notch) within target cis-regulatory elements might be a general mechanism of organ-field specification in vertebrates (as it is in Drosophila). PMID: 18057103
Subcellular Location Nucleus
Protein Families Paired homeobox family, Bicoid subfamily
Database Links

KEGG: xtr:548931

STRING: 8364.ENSXETP00000034219

UniGene: Str.10021

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