Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

yscM

Uniprot No.

P69979

Research Area

Microbiology

Alternative Names

yscM; lcrQ; pYV0089; Yop proteins translocation protein M; Low calcium response locus protein Q

Species

Yersinia pseudotuberculosis serotype I (strain IP32953)

Source

E.coli

Expression Region

1-115aa

Target Protein Sequence

MKINTLQSLINQQITQVGHGGQAGRLTETNPLTENSHQISTAEKAFANEVLEHVKNTALSRHDIACLLPRVSNLELKQGKAGEVIVTGLRTEQLSLSDAKLLLEAAMRQDTAADG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.4kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Y.pseudotuberculosis serotype I yscM protein is an E.coli-expressed Full Length protein. Molecularly, this yscM protein is characterized by N-terminal 6xHis-SUMO tag, internal Y.pseudotuberculosis serotype I yscM DNA fragment (1-115aa). Standard methods for recombinant yscM protein expression comprise transfecting cells with DNA vectors that consist of specific templates and then cultures cells to translate and transcribe the yscM protein production process. Typically, these cells are lysed to extract expressed proteins for more purification. The purity of this recombinant yscM protein is 90%+ measured by SDS-PAGE.

yscM, also called LcrQ, is a regulatory protein specific to Yersinia. LcrQ acts as an anti-activator that causes feedback inhibition for Ysc-Yop type III secretion system (T3SS) gene expression. LcrQ is secreted outside of cells by the secretory machinery under low calcium conditions at 37 °C with ligation to the chaperone protein SycH, easing the inhibitory effect of LcrQ and leading to the activation of the Ysc-Yop T3SS. LcrQ-SycH interaction also promotes the T3SS of LcrQ to the outside environment, further increasing Yop synthesis and secretion.

Target Background

Function

Belongs to an operon involved in the translocation of Yop proteins across the bacterial membranes or in the specific control of this function.

Database Links

KEGG: ypo:BZ17_4246

Code	CSB-EP300275YAH
Abbreviation	Recombinant Yersinia pseudotuberculosis serotype I yscM protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Yersinia pseudotuberculosis serotype I Yop proteins translocation protein M (yscM)

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