Code | CSB-BP522532ZAS |
Abbreviation | Recombinant Zaire ebolavirus VP24 protein |
MSDS | |
Size | $528 |
Order now | |
Image | |
Have Questions? | Leave a Message or Start an on-line Chat |
The gene responsible for the Zaire ebolavirus (strain Gabon-94) VP24 protein (1-251aa) is cloned into a plasmid vector to obtain recombinant plasmid, which is then transformed into baculovirus cells. baculovirus cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to endure a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. Post-expression, affinity purification is employed to isolate and purify the recombinant Zaire ebolavirus (strain Gabon-94) VP24 protein from the cell lysate. The resulting recombinant protein is subjected to denaturing SDS-PAGE, allowing for an estimation of its purity, surpassing 85%.
There are currently no reviews for this product.